Rabbit SETD2 Polyclonal Antibody | anti-SETD2 antibody

SETD2 (SET Domain-containing Protein 2, Histone-lysine N-methyltransferase SETD2, HYPB, SET2, HIF-1, HIP-1, KMT3A, HBP231, HSPC069, p231HBP) (MaxLight 750)

Cat. Num. AAA6437219

• Gene Names: SETD2; HYPB; SET2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Purified by affinity chromatography.
• Synonyms: SETD2; Polyclonal Antibody; SETD2 (SET Domain-containing Protein 2; Histone-lysine N-methyltransferase SETD2; HYPB; SET2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP) (MaxLight 750); anti-SETD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SETD2. Species Crossreactivity: mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-SETD2 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; IP: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human SETD2.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for anti-SETD2 antibody

Antibodies; Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 296452963
• NCBI GeneID: 29072
• UniProt Accession #: Q9BYW2; O75397; O75405; Q17RW8; Q5BKS9; Q5QGN2; Q69YI5; Q6IN64; Q6ZN53; Q6ZS25; Q8N3R0; Q8TCN0
• Molecular Weight: 2564
• NCBI Official Full Name: Histone-lysine N-methyltransferase SETD2
• NCBI Official Synonym Full Names: SET domain containing 2
• NCBI Official Symbol: SETD2
• NCBI Official Synonym Symbols: HYPB; SET2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP
• NCBI Protein Information: histone-lysine N-methyltransferase SETD2; huntingtin yeast partner B; lysine N-methyltransferase 3A; huntingtin interacting protein 1; huntingtin-interacting protein B
• UniProt Protein Name: Histone-lysine N-methyltransferase SETD2
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SETD2
• UniProt Synonym Gene Names: HIF1; HYPB; KIAA1732; KMT3A; SET2; HIP-1; hSET2
• UniProt Entry Name: SETD2_HUMAN

NCBI Description

Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II. [provided by RefSeq, Aug 2008]

Uniprot Description

SETD2: Histone methyltransferase that methylates 'Lys-36' of histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. Binds DNA at promoters. May also act as a transcription activator that binds to promoters. Binds to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression. Belongs to the histone-lysine methyltransferase family. SET2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine; EC 2.1.1.43; Methyltransferase

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: nucleoplasm; chromosome

Molecular Function: protein binding; histone-lysine N-methyltransferase activity

Biological Process: pericardium development; establishment and/or maintenance of chromatin architecture; stem cell development; mismatch repair; regulation of transcription, DNA-dependent; mesoderm morphogenesis; forebrain development; neural tube closure; RNA elongation from RNA polymerase II promoter; embryonic cranial skeleton morphogenesis; morphogenesis of a branching structure; angiogenesis