Rabbit PSMD3 Polyclonal Antibody | anti-PSMD3 antibody

PSMD3 (Proteasome 26S Subunit, Non ATPase 3, S3, P58, RPN3, TSTA2) (PE)

Cat. Num. AAA6433711

• Gene Names: PSMD3; S3; P58; RPN3; TSTA2
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: PSMD3; Polyclonal Antibody; PSMD3 (Proteasome 26S Subunit; Non ATPase 3; S3; P58; RPN3; TSTA2) (PE); anti-PSMD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PSMD3. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PSMD3 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human PSMD3.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PSMD3 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thyroid cancer tissue using PSMD3 antibody.)

Product Categories/Family for anti-PSMD3 antibody

Antibodies; Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 25777612
• NCBI GeneID: 5709
• NCBI Accession #: NP_002800.2
• NCBI GenBank Nucleotide #: NM_002809.3
• UniProt Accession #: O43242; Q96EI2; Q9BQA4; B3KMW9
• Molecular Weight: 60,978 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 3
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
• NCBI Official Symbol: PSMD3
• NCBI Official Synonym Symbols: S3; P58; RPN3; TSTA2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 3; proteasome subunit p58; 26S proteasome regulatory subunit S3; 26S proteasome regulatory subunit RPN3; tissue specific transplantation antigen 2
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 3
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD3
• UniProt Entry Name: PSMD3_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid. Single nucleotide polymorphisms in this gene are associated with neutrophil count. [provided by RefSeq, Jul 2012]

Uniprot Description

PSMD3: Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Belongs to the proteasome subunit S3 family.

Protein type: Proteasome complex; Protease

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: nucleoplasm; proteasome complex; membrane; cytoplasm; nucleus; cytosol

Molecular Function: enzyme regulator activity

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis