Rabbit PRKCQ Polyclonal Antibody | anti-PRKCQ antibody

PRKCQ, Phosphorylated (S695) (Protein Kinase C, theta, PRKCT, nPKC-theta) (HRP)

Cat. Num. AAA6433034

• Gene Names: PRKCQ; PRKCT; nPKC-theta
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: PRKCQ; Polyclonal Antibody; Phosphorylated (S695) (Protein Kinase C; theta; PRKCT; nPKC-theta) (HRP); anti-PRKCQ antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PRKCQ, phosphorylated S695. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PRKCQ antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding S695 of human PRKCQ.
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, using Phospho-PRKCQ-S695 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Phospho-PRKCQ-S695 antibody.)

Product Categories/Family for anti-PRKCQ antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 334848126
• NCBI GeneID: 5588
• NCBI Accession #: NP_001229342.1
• NCBI GenBank Nucleotide #: NM_001242413.1
• UniProt Accession #: Q04759; Q14DH6; Q3MJF1; Q64FY5; Q9H508; Q9H549
• Molecular Weight: 81,865 Da
• NCBI Official Full Name: protein kinase C theta type isoform 2
• NCBI Official Synonym Full Names: protein kinase C, theta
• NCBI Official Symbol: PRKCQ
• NCBI Official Synonym Symbols: PRKCT; nPKC-theta
• NCBI Protein Information: protein kinase C theta type
• UniProt Protein Name: Protein kinase C theta type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCQ
• UniProt Synonym Gene Names: PRKCT
• UniProt Entry Name: KPCT_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCT: an AGC kinase of the PKC family. A novel PKC: Ca2+ independent but still regulated by PS, DAG and phorbol esters. Contains 2 zinc-dependent phorbol-ester and DAG binding domains. Expressed in skeletal muscle and hematopoietic cells. May play a role in T cell receptor signaling and insulin resistance. Required for TCR-induced NF-kappaB activation in mature T lymphocytes.

Protein type: Kinase, protein; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; PKC family; Delta subfamily

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: plasma membrane; immunological synapse; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ubiquitin-protein ligase activity; ATP binding

Biological Process: positive regulation of interleukin-17 production; axon guidance; phototransduction, visible light; platelet activation; apoptosis; positive regulation of interleukin-2 biosynthetic process; membrane protein ectodomain proteolysis; negative regulation of insulin receptor signaling pathway; protein ubiquitination; protein amino acid phosphorylation; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; rhodopsin mediated signaling; positive regulation of interleukin-4 production; regulation of rhodopsin mediated signaling; regulation of transcription, DNA-dependent; innate immune response; positive regulation of T cell proliferation; regulation of cell growth; positive regulation of T cell activation; inflammatory response; blood coagulation; cell structure disassembly during apoptosis