Rabbit anti-Human MAX Polyclonal Antibody | anti-MAX antibody

MAX, Phosphorylated (pS11) (MYC Associated Factor X, MGC10775, MGC11225, MGC18164, MGC34679, MGC36767,bHLHd4,_+E_bHLHd5, bHLHd6, bHLHd7, bHLHd8, orf1) (HRP)

Cat. Num. AAA6426148

• Gene Names: MAX; bHLHd4
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: MAX; Polyclonal Antibody; Phosphorylated (pS11) (MYC Associated Factor X; MGC10775; MGC11225; MGC18164; MGC34679; MGC36767; bHLHd4; _+E_bHLHd5; bHLHd6; bHLHd7; bHLHd8; orf1) (HRP); anti-MAX antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAX, phosphorylated pS11.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-MAX antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding S11 of_+E_human MAX.
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using Phospho-MAX-S11 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-MAX antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 47117704
• NCBI GeneID: 4149
• UniProt Accession #: P61244; P25912; P52163; Q14803; Q96CY8; A6NH73; A8K265; A8K4G4; A8K824
• Molecular Weight: 160
• NCBI Official Full Name: Protein max
• NCBI Official Synonym Full Names: MYC associated factor X
• NCBI Official Symbol: MAX
• NCBI Official Synonym Symbols: bHLHd4
• NCBI Protein Information: protein max; class D basic helix-loop-helix protein 4
• UniProt Protein Name: Protein max
• Protein Family: Maximin
• UniProt Gene Name: MAX
• UniProt Synonym Gene Names: BHLHD4; bHLHd4
• UniProt Entry Name: MAX_HUMAN

NCBI Description

The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Mutations of this gene have been reported to be associated with hereditary pheochromocytoma. A pseudogene of this gene is located on the long arm of chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

MAX: a transcription factor. Forms a DNA- binding protein complex with MYC or MAD. The MYC-MAX complex is a transcriptional activator, whereas the MAD-MAX complex is a repressor. CPG methylation of the recognition site greatly inhibits DNA binding, suggesting that DNA methylation may regulate the MYC/MAX complex in vivo. May repress transcription via the recruitment of a chromatin remodeling complex containing H3-K9 histone methyltransferase activity. Three alternatively spliced isoforms have been reported.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 14q23

Cellular Component: nucleoplasm; PML body; dendrite; cytoplasm; nucleus

Molecular Function: protein binding; protein homodimerization activity; sequence-specific DNA binding; protein heterodimerization activity; transcription coactivator activity; transcription cofactor activity; protein complex binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; neuron apoptosis; regulation of transcription, DNA-dependent; retina development in camera-type eye; protein complex assembly; response to axon injury; cellular response to starvation; response to insulin stimulus