Rabbit KIF2C Polyclonal Antibody | anti-KIF2C antibody

KIF2C (Kinesin 2C, MCAK, KNSL6) (PE)

Cat. Num. AAA6423855

• Gene Names: KIF2C; MCAK; KNSL6
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: KIF2C; Polyclonal Antibody; KIF2C (Kinesin 2C; MCAK; KNSL6) (PE); anti-KIF2C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes KIF2C. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-KIF2C antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human KIF2C.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using KIF2C antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using KIF2C antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophageal cancer using KIF2C antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using KIF2C antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using KIF2C antibody at dilution of 1:200 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using KIF2C antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-KIF2C antibody

Antibodies; Motor Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 166795250
• NCBI GeneID: 11004
• NCBI Accession #: NP_006836.2
• NCBI GenBank Nucleotide #: NM_006845.3
• UniProt Accession #: Q99661; Q5JR88; Q6ICU1; Q96C18; Q96HB8; Q9BWV8; B3ITR9
• Molecular Weight: 81,313 Da
• NCBI Official Full Name: kinesin-like protein KIF2C
• NCBI Official Synonym Full Names: kinesin family member 2C
• NCBI Official Symbol: KIF2C
• NCBI Official Synonym Symbols: MCAK; KNSL6
• NCBI Protein Information: kinesin-like protein KIF2C; kinesin-like 6; kinesin-like protein 6; mitotic centromere-associated kinesin
• UniProt Protein Name: Kinesin-like protein KIF2C
• Protein Family: Kinesin-like protein
• UniProt Gene Name: KIF2C
• UniProt Synonym Gene Names: KNSL6; MCAK
• UniProt Entry Name: KIF2C_HUMAN

NCBI Description

The protein encoded by this gene is a member of kinesin-like protein family. Proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation. [provided by RefSeq, Jul 2008]

Uniprot Description

KIF2C: In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA); Microtubule-binding; Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: kinetochore; microtubule cytoskeleton; kinesin complex; membrane; cytoplasmic microtubule; cytosol; nucleus; chromosome, pericentric region

Molecular Function: centromeric DNA binding; microtubule plus-end binding; protein binding; ATPase activity; microtubule motor activity; ATP binding

Biological Process: mitosis; cell proliferation; cell division; metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; microtubule depolymerization; regulation of chromosome segregation; mitotic cell cycle; blood coagulation; mitotic metaphase plate congression; microtubule-based movement; establishment and/or maintenance of microtubule cytoskeleton polarity