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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using DDX21 antibody.)

Rabbit anti-Human DDX21 Polyclonal Antibody | anti-DDX21 antibody

DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21, GUA, GURDB, RH-II/GU, RH-II/GuA) (Biotin)

Gene Names
DDX21; GUA; GURDB; RH-II/GU; RH-II/GuA
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation
Purity
Purified by affinity chromatography.
Synonyms
DDX21; Polyclonal Antibody; DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21; GUA; GURDB; RH-II/GU; RH-II/GuA) (Biotin); anti-DDX21 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human DDX21.
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-DDX21 antibody
Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
ICC: 1:50-1:200
WB: 1:500-1:1000
IP: 1:20-1:50
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human DDX21.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using DDX21 antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using DDX21 antibody.)
Product Categories/Family for anti-DDX21 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
783
NCBI Official Full Name
Nucleolar RNA helicase 2
NCBI Official Synonym Full Names
DEAD (Asp-Glu-Ala-Asp) box helicase 21
NCBI Official Symbol
DDX21
NCBI Official Synonym Symbols
GUA; GURDB; RH-II/GU; RH-II/GuA
NCBI Protein Information
nucleolar RNA helicase 2; RH II/Gu; gu-alpha; Gu protein; DEAD box protein 21; RNA helicase II/Gu alpha; nucleolar RNA helicase Gu; nucleolar RNA helicase II; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21
UniProt Protein Name
Nucleolar RNA helicase 2
Protein Family
UniProt Gene Name
DDX21
UniProt Entry Name
DDX21_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX21: a protein of the DEAD box helicase family. Unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.

Protein type: EC 3.6.4.13; RNA processing; Helicase; Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 10q21

Cellular Component: membrane; nucleolus; nucleus

Molecular Function: double-stranded RNA binding; ATP-dependent RNA helicase activity; ATP binding

Biological Process: osteoblast differentiation; response to exogenous dsRNA; metabolic process; response to virus

Research Articles on DDX21

Similar Products

Product Notes

The DDX21 ddx21 (Catalog #AAA6413826) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21, GUA, GURDB, RH-II/GU, RH-II/GuA) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DDX21 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP). ICC: 1:50-1:200 WB: 1:500-1:1000 IP: 1:20-1:50 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DDX21 ddx21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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