Rabbit ADRA1A Polyclonal Antibody | anti-ADRA1A antibody

ADRA1A (Adrenergic Receptor Alpha 1A, ADRA1C, ADRA1L1, ALPHA1AAR) (Biotin)

Cat. Num. AAA6405675

• Gene Names: ADRA1A; ADRA1C; ADRA1L1; ALPHA1AAR
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: ADRA1A; Polyclonal Antibody; ADRA1A (Adrenergic Receptor Alpha 1A; ADRA1C; ADRA1L1; ALPHA1AAR) (Biotin); anti-ADRA1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes ADRA1A. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-ADRA1A antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 1:50-1:100; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human ADRA1A.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of Jurkat cell line, using ADRA1A antibody.)

Product Categories/Family for anti-ADRA1A antibody

Antibodies; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 111118992
• NCBI GeneID: 148
• NCBI Accession #: NP_000671.2
• NCBI GenBank Nucleotide #: NM_000680.2
• UniProt Accession #: P35348; O60451; Q13675; Q13729; A8K0I3; B0ZBD1; B0ZBD2; B0ZBD4; B0ZBD5; B0ZBD6; B0ZBD8; B0ZBD9
• Molecular Weight: 51,487 Da
• NCBI Official Full Name: alpha-1A adrenergic receptor isoform 1
• NCBI Official Synonym Full Names: adrenoceptor alpha 1A
• NCBI Official Symbol: ADRA1A
• NCBI Official Synonym Symbols: ADRA1C; ADRA1L1; ALPHA1AAR
• NCBI Protein Information: alpha-1A adrenergic receptor; alpha-1A adrenoceptor; alpha-1A adrenoreceptor; G protein coupled receptor; alpha-1C adrenergic receptor; adrenergic, alpha-1A-, receptor variant 1; adrenergic, alpha-1A-, receptor variant 3; adrenergic, alpha-1A-, receptor v
• UniProt Protein Name: Alpha-1A adrenergic receptor
• Protein Family: Alpha-1A adrenergic receptor
• UniProt Gene Name: ADRA1A
• UniProt Synonym Gene Names: ADRA1C; Alpha-1A adrenoceptor
• UniProt Entry Name: ADA1A_HUMAN

NCBI Description

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008]

Uniprot Description

ADRA1A: This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: nuclear membrane; integral to plasma membrane; T-tubule; plasma membrane; nucleus; Z disc

Molecular Function: protein heterodimerization activity; alpha1-adrenergic receptor activity

Biological Process: apoptosis; response to hormone stimulus; positive regulation of systemic arterial blood pressure; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; signal transduction; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; smooth muscle contraction; positive regulation of MAPKKK cascade; cell-cell signaling; positive regulation of smooth muscle contraction; positive regulation of action potential; cell growth; aging; response to drug; adult heart development; positive regulation of the force of heart contraction by epinephrine-norepinephrine; positive regulation of synaptic transmission, GABAergic; micturition; organ growth; negative regulation of heart rate in baroreceptor response to increased systemic arterial blood pressure; G-protein coupled receptor protein signaling pathway; negative regulation of Rho protein signal transduction; phospholipase C activation; positive regulation of vasoconstriction; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of heart rate by epinephrine-norepinephrine