Rabbit anti-Human, Mouse STAT6 Polyclonal Antibody | anti-STAT6 antibody

STAT6 (Signal Transducer and Activator of Transcription 6, IL-4 Stat) (HRP)

Cat. Num. AAA6395283

• Gene Names: STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: STAT6; Polyclonal Antibody; STAT6 (Signal Transducer and Activator of Transcription 6; IL-4 Stat) (HRP); anti-STAT6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human STAT6. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-STAT6 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human STAT6, aa1-847 (NP_003144.3).
• Immunogen Sequence: MSLWGLVSKMPPEKVQRLYVDFPQHLRHLLGDWLESQPWEFLVGSDAFCCNLASALLSDTVQHLQASVGEQGEGSTILQHISTLESIYQRDPLKLVATFRQILQGEKKAVMEQFRHLPMPFHWKQEELKFKTGLRRLQHRVGEIHLLREALQKGAEAGQVSLHSLIETPANGTGPSEALAMLLQETTGELEAAKALVLKRIQIWKRQQQLAGNGAPFEESLAPLQERCESLVDIYSQLQQEVGAAGGELEPKTRASLTGRLDEVLRTLVTSCFLVEKQPPQVLKTQTKFQAGVRFLLGLRFLGAPAKPPLVRADMVTEKQARELSVPQGPGAGAESTGEIINNTVPLENSIPGNCCSALFKNLLLKKIKRCERKGTESVTEEKCAVLFSASFTLGPGKLPIQLQALSLPLVVIVHGNQDNNAKATILWDNAFSEMDRVPFVVAERVPWEKMCETLNLKFMAEVGTNRGLLPEHFLFLAQKIFNDNSLSMEAFQHRSVSWSQFNKEILLGRGFTFWQWFDGVLDLTKRCLRSYWSDRLIIGFISKQYVTSLLLNEPDGTFLLRFSDSEIGGITIAHVIRGQDGSPQIENIQPFSAKDLSIRSLGDRIRDLAQLKNLYPKKPKDEAFRSHYKPEQMGKDGRGYVPATIKMTVERDQPLPTPELQMPTMVPSYDLGMAPDSSMSMQLGPDMVPQVYPPHSHSIPPYQGLSPEESVNVLSAFQEPHLQMPPSLGQMSLPFDQPHPQGLLPCQPQEHAVSSPDPLLCSDVTMVEDSCLSQPVTAFPQGTWIGEDIFPPLLPPTEQDLTKLLLEGQGESGGGSLGAQPLLQPSHYGQSGISMSHMDLRANPSW
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(STAT6 rabbit polyclonal antibody. Western Blot analysis of STAT6 expression in mouse brain.)

Western Blot (WB)

(STAT6 rabbit polyclonal antibody. Western Blot analysis of STAT6 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of STAT6 expression in transfected 293T cell line by STAT6 polyclonal antibody. Lane 1: STAT6 transfected lysate (94.1kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-STAT6 antibody

Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13. In addition, Stat6 is activated by IFNa in B cell lines, where it forms transcriptionally active complexes with Stat2 and p48. Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling.

Product Categories/Family for anti-STAT6 antibody

Antibodies; Transcription Factors; STAT

NCBI and Uniprot Product Information

• NCBI GI #: 23397678
• NCBI GeneID: 6778
• NCBI Accession #: NP_003144.3
• NCBI GenBank Nucleotide #: NM_003153.3
• UniProt Accession #: P42226; Q5FBW5; Q71UP4; A8K316; B7ZA27; F5GXI9
• Molecular Weight: 94,135 Da
• NCBI Official Full Name: signal transducer and activator of transcription 6 isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 6, interleukin-4 induced
• NCBI Official Symbol: STAT6
• NCBI Official Synonym Symbols: STAT6B; STAT6C; D12S1644; IL-4-STAT
• NCBI Protein Information: signal transducer and activator of transcription 6; STAT, interleukin4-induced; transcription factor IL-4 STAT
• UniProt Protein Name: Signal transducer and activator of transcription 6
• Protein Family: Signal transducer and transcription activator
• UniProt Gene Name: STAT6
• UniProt Entry Name: STAT6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

STAT6: transcription factor of the STAT family. Plays a central role in IL4-mediated biological responses. Induces the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. May function in the differentiation of T helper 2 cells and class switch of immunoglobulins. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; cytosol; lipid raft

Molecular Function: identical protein binding; signal transducer activity; protein binding; transcription factor activity; protein phosphatase binding

Biological Process: regulation of transcription from RNA polymerase II promoter; T-helper 1 cell lineage commitment; negative regulation of T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interferon type I production; innate immune response; mammary gland epithelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of isotype switching to IgE isotypes; regulation of cell proliferation