Rabbit anti-Human RING Finger Protein 168 Polyclonal Antibody | anti-RNF168 antibody

RING Finger Protein 168 (RNF168, E3 Ubiquitin-protein Ligase RNF168, hRNF168) (FITC)

Cat. Num. AAA6392730

• Gene Names: RNF168; hRNF168
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RING Finger Protein 168; Polyclonal Antibody; RING Finger Protein 168 (RNF168; E3 Ubiquitin-protein Ligase RNF168; hRNF168) (FITC); EC=6.3.2.-; anti-RNF168 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RNF168.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RNF168 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human RNF168, aa1-571 (NP_689830.2).
• Immunogen Sequence: MALPKDAIPSLSECQCGICMEILVEPVTLPCNHTLCKPCFQSTVEKASLCCPFCRRRVSSWTRYHTRRNSLVNVELWTIIQKHYPRECKLRASGQESEEVADDYQPVRLLSKPGELRREYEEEISKVAAERRASEEEENKASEEYIQRLLAEEEEEEKRQAEKRRRAMEEQLKSDEELARKLSIDINNFCEGSISASPLNSRKSDPVTPKSEKKSKNKQRNTGDIQKYLTPKSQFGSASHSEAVQEVRKDSVSKDIDSSDRKSPTGQDTEIEDMPTLSPQISLGVGEQGADSSIESPMPWLCACGAEWYHEGNVKTRPSNHGKELCVLSHERPKTRVPYSKETAVMPCGRTESGCAPTSGVTQTNGNNTGETENEESCLLISKEISKRKNQESSFEAVKDPCFSAKRRKVSPESSPDQEETEINFTQKLIDLEHLLFERHKQEEQDRLLALQLQKEVDKEQMVPNRQKGSPDEYHLRATSSPPDKVLNGQRKNPKDGNFKRQTHTKHPTPERGSRDKNRQVSLKMQLKQSVNRRKMPNSTRDHCKVSKSAHSLQPSISQKSVFQMFQRCTK
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RNF168 rabbit polyclonal antibody. Western Blot analysis of RNF168 expression in human liver.)

Western Blot (WB)

(Western Blot analysis of RNF168 expression in transfected 293T cell line by RNF168 polyclonal antibody. Lane 1: RNF168 transfected lysate (65.0kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-RNF168 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 31377566
• NCBI GeneID: 165918
• NCBI Accession #: NP_689830.2
• NCBI GenBank Nucleotide #: NM_152617.2
• UniProt Accession #: Q8IYW5; Q8NA67; Q96NS4
• Molecular Weight: 65,020 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF168
• NCBI Official Synonym Full Names: ring finger protein 168, E3 ubiquitin protein ligase
• NCBI Official Symbol: RNF168
• NCBI Official Synonym Symbols: hRNF168
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF168
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF168
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF168
• UniProt Synonym Gene Names: hRNF168
• UniProt Entry Name: RN168_HUMAN

Uniprot Description

RNF168: E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8- dependent H2A ubiquitination, promoting the formation of 'Lys-63'- linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively). Defects in RNF168 are the cause of Riddle syndrome (RIDDLES). Riddle syndrome is characterized by increased radiosensitivity, immunodeficiency, mild motor control and learning difficulties, facial dysmorphism, and short stature. Defects are probably due to impaired localization of TP53BP1 and BRCA1 at DNA lesions. Belongs to the RNF168 family.

Protein type: Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: nucleoplasm; cytoplasm; nucleus; ubiquitin ligase complex

Molecular Function: ubiquitin binding; protein binding; histone binding; zinc ion binding; nucleosome binding; ubiquitin-protein ligase activity; chromatin binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of DNA repair; double-strand break repair; isotype switching; protein ubiquitination; response to ionizing radiation; response to DNA damage stimulus

Disease: Riddle Syndrome