Rabbit anti-Human, Mouse RBM5 Polyclonal Antibody | anti-RBM5 antibody

RBM5 (RNA-binding Protein 5, RNA-binding Motif Protein 5, Putative Tumor Suppressor LUCA15, Protein G15, Renal Carcinoma Antigen NY-REN-9, H37, LUCA15) (HRP)

Cat. Num. AAA6392148

• Gene Names: RBM5; G15; H37; RMB5; LUCA15
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RBM5; Polyclonal Antibody; RBM5 (RNA-binding Protein 5; RNA-binding Motif Protein 5; Putative Tumor Suppressor LUCA15; Protein G15; Renal Carcinoma Antigen NY-REN-9; H37; LUCA15) (HRP); anti-RBM5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RBM5. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RBM5 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full-length human RBM5, aa1-546 (AAH02957.1).
• Immunogen Sequence: MGSDKRVSRTERSGRYGSIIDRDDRDERESRSRRRDSDYKRSSDDRRGDRYDDYRDYDSPERERERRNSDRSEDGYHSDGDYGEHDYRHDISDERESKTIMLRGLPITITESDIREMMESFEGPQPADVRLMKRKTGVSRGFAFVEFYHLQDATSWMEANQKKLVIQGKHIAMHYSNPRPKFEDWLCNKCCLNNFRKRLKCFRCGADKFDSEQEVPPGTTESVQSVDYYCDTIILRNIAPHTVVDSIMTALSPYASLAVNNIRLIKDKQTQQNRGFAFVQLSSAMDASQLLQILQSLHPPLKIDGKTIGVDFAKSARKDLVLSDGNRVSAFSVASTAIAAAQWSSTQSQSGEGGSVDYSYLQPGQDGYAQYAQYSQDYQQFYQQQAGGLESDASSASGTAVTTTSAAVVSQSPQLYNQTSNPPGSPTEEAQPSTSTSTQAPAASPTGVVPGTKYAVPDTSTYQYDESSGYYYDPTTGLYYDPNSQYYYNSLTQQYLYWDGEKETYVPAAESSSHQQSGLPPAKEGKEKKEKPKSKTAQQIAKDMER
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RBM5 rabbit polyclonal antibody. Western Blot analysis of RBM5 expression in human placenta.)

Western Blot (WB)

(RBM5 rabbit polyclonal antibody. Western Blot analysis of RBM5 expression in mouse kidney.)

Western Blot (WB)

(Western Blot analysis of RBM5 expression in transfected 293T cell line by RBM5 polyclonal antibody. Lane 1: RBM5 transfected lysate (61.5kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-RBM5 antibody

Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.

Product Categories/Family for anti-RBM5 antibody

Antibodies; RNA Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 12804198
• NCBI GeneID: 10181
• Molecular Weight: 17,891 Da
• NCBI Official Full Name: Homo sapiens RNA binding motif protein 5, mRNA
• NCBI Official Synonym Full Names: RNA binding motif protein 5
• NCBI Official Symbol: RBM5
• NCBI Official Synonym Symbols: G15; H37; RMB5; LUCA15
• NCBI Protein Information: RNA-binding protein 5
• Protein Family: RNA-binding protein

NCBI Description

This gene is a candidate tumor suppressor gene which encodes a nuclear RNA binding protein that is a component of the spliceosome A complex. The encoded protein plays a role in the induction of cell cycle arrest and apoptosis through pre-mRNA splicing of multiple target genes including the tumor suppressor protein p53. This gene is located within the tumor suppressor region 3p21.3, and may play a role in the inhibition of tumor transformation and progression of several malignancies including lung cancer. [provided by RefSeq, Oct 2011]