Thyroglobulin Steroid
Thyroglobulin, Human (Tg)
Applications
ELISA
Purity
Purified90% by SDS-PAGE. Immunological identity confirmed by reaction with monoclonal antibody that is specific for human thyroglobulin.
Synonyms
Thyroglobulin; Human (Tg); Thyroglobulin steroid
Host
Human thyroid gland
Purity/Purification
Purified
90% by SDS-PAGE. Immunological identity confirmed by reaction with monoclonal antibody that is specific for human thyroglobulin.
90% by SDS-PAGE. Immunological identity confirmed by reaction with monoclonal antibody that is specific for human thyroglobulin.
Form/Format
Supplied as a lyophilized powder from 25mM Tris-HCl, pH 8.0. Reconstitute with sterile ddH2O.
Applicable Applications for Thyroglobulin steroid
ELISA (EL/EIA)
Application Notes
Suitable for use as a standard in immunoassays for determination of serum thyroglobulin levels, immunogen for antisera production, tracer for iodination.
CAS Number
9010-34-8
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Thyroglobulin steroid
Thyroglobulin (TG) is the primary protein substrate in the formation of thyroid hormone. It accounts for the major portion of the thyroid gland mass. TG consists of a dimer of 2 identical proteins in a noncovalent linkage. Each monomer contains 2748 aa. TG has a MW of ~330kD, ~10% of the weight is due to glycosylation. The peptide chains may be found in monomeric and dimeric forms. TG is synthesized exclusively by the thyroid follicular cells. It is stored within the thyroid follicles where it comprises most of the thyroid colloid. The TG molecule contains ~110 tyrosine residues which form the substrate for thyroid hormone formation. Through an active transport mechanism, the thyroid gland traps iodide, which is oxidized to iodine within the thyroid follicles. Iodination of TG on tyrosine residues then occurs, resulting in the formation of mono- and di-iodotyrosine (MIT and DIT). MIT and DIT are then proteolytically cleaved from TG and are coupled to form the active thyroid hormones: triiodothyronine (MIT+DIT, T3) and thyroxine (DIT+DIT, T4). Both iodination and coupling are mediated by a peroxidase enzyme. The ratio of DIT to MIT in TG is ~2:1, while the final ratio of T4:T3 within the thyroid gland is ~3:1. T4 and T3 are then released into the bloodstream along with small amounts of TG. Thyroglobulin synthesis is stimulated at the transcriptional level by thyroid-stimulating hormone (TSH). Serum thyroglobulin levels are relatively high during late gestation and in cord blood. Levels decline during childhood and adolescence. Levels are slightly higher in term newborn females as compared to males.
Product Categories/Family for Thyroglobulin steroid
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Product Notes
The Thyroglobulin (Catalog #AAA638988) is a Steroid produced from Human thyroid gland and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Thyroglobulin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use as a standard in immunoassays for determination of serum thyroglobulin levels, immunogen for antisera production, tracer for iodination. Researchers should empirically determine the suitability of the Thyroglobulin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thyroglobulin, Steroid" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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