Rabbit anti-Human, Mouse NALP2 Polyclonal Antibody | anti-NALP2 antibody

NALP2 (NACHT, LRR and PYD Domains-containing Protein 2, Nucleotide-binding site Protein 1, PYRIN Domain and NACHT Domain-containing Protein 1, PYRIN-containing APAF1-like Protein 2, NLRP2, NBS1, PAN1, PYPAF2, CLR19.9, FLJ20510) (HRP)

Cat. Num. AAA6387121

• Gene Names: NLRP2; NBS1; PAN1; NALP2; PYPAF2; CLR19.9
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NALP2; Polyclonal Antibody; NALP2 (NACHT; LRR and PYD Domains-containing Protein 2; Nucleotide-binding site Protein 1; PYRIN Domain and NACHT Domain-containing Protein 1; PYRIN-containing APAF1-like Protein 2; NLRP2; NBS1; PAN1; PYPAF2; CLR19.9; FLJ20510) (HRP); anti-NALP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NLRP2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NALP2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human NLRP2, aa1-1062 (NP_060322.1).
• Immunogen Sequence: MVSSAQMGFNLQALLEQLSQDELSKFKYLITTFSLAHELQKIPHKEVDKADGKQLVEILTTHCDSYWVEMASLQVFEKMHRMDLSERAKDEVREAALKSFNKRKPLSLGITRKERPPLDVDEMLERFKTEAQAFTETKGNVICLGKEVFKGKKPDKDNRCRYILKTKFREMWKSWPGDSKEVQVMAERYKMLIPFSNPRVLPGPFSYTVVLYGPAGLGKTTLAQKLMLDWAEDNLIHKFKYAFYLSCRELSRLGPCSFAELVFRDWPELQDDIPHILAQARKILFVIDGFDELGAAPGALIEDICGDWEKKKPVPVLLGSLLNRVMLPKAALLVTTRPRALRDLRILAEEPIYIRVEGFLEEDRRAYFLRHFGDEDQAMRAFELMRSNAALFQLGSAPAVCWIVCTTLKLQMEKGEDPVPTCLTRTGLFLRFLCSRFPQGAQLRGALRTLSLLAAQGLWAQTSVLHREDLERLGVQESDLRLFLDGDILRQDRVSKGCYSFIHLSFQQFLTALFYTLEKEEEEDRDGHTWDIGDVQKLLSGVERLRNPDLIQAGYYSFGLANEKRAKELEATFGCRMSPDIKQELLRCDISCKGGHSTVTDLQELLGCLYESQEEELVKEVMAQFKEISLHLNAVDVVPSSFCVKHCRNLQKMSLQVIKENLPENVTASESDAEVERSQDDQHMLPFWTDLCSIFGSNKDLMGLAINDSFLSASLVRILCEQIASDTCHLQRVVFKNISPADAHRNLCLALRGHKTVTYLTLQGNDQDDMFPALCEVLRHPECNLRYLGLVSCSATTQQWADLSLALEVNQSLTCVNLSDNELLDEGAKLLYTTLRHPKCFLQRLSLENCHLTEANCKDLAAVLVVSRELTHLCLAKNPIGNTGVKFLCEGLRYPECKLQTLVLWNCDITSDGCCDLTKLLQEKSSLLCLDLGLNHIGVKGMKFLCEALRKPLCNLRCLWLWGCSIPPFSCEDLCSALSCNQSLVTLDLGQNPLGSSGVKMLFETLTCSSGTLRTLRLKIDDFNDELNKLLEEIEEKNPQLIIDTEKHHPWAERPSSHDFMI
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NLRP2 rabbit polyclonal antibody. Western Blot analysis of NLRP2 expression in human pancreas.)

Western Blot (WB)

(NLRP2 rabbit polyclonal antibody. Western Blot analysis of NLRP2 expression in mouse spleen.)

Western Blot (WB)

(Western Blot analysis of NLRP2 expression in transfected 293T cell line by NLRP2 polyclonal antibody. Lane 1: NLRP2 transfected lysate (120.5kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-NALP2 antibody

NALP2 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. Unlike the prototypical NALP protein NALP1, NALP2 only contains a NACHT domain, leucine rich repeat (LRR), and pyrin-containing domain (PYD). This protein interacts with the adapter protein ASC in addition to CARD8 and caspase-1 to form an inflammasome with high proIL-1b-processing activity and is thought to function as a modulator of NF-kB and procaspase-1 activation in macrophages. It has also been suggested that NALP2, in addition to other NALP family members, can act as innate sensors of pathogens in a manner similar to the toll-like receptors (TLRs). At least two alternatively spliced transcript variants encoding distinct isoforms have been found for NALP2.

Product Categories/Family for anti-NALP2 antibody

Antibodies; Inflammasomes

NCBI and Uniprot Product Information

• NCBI GI #: 8923473
• NCBI GeneID: 55655
• NCBI Accession #: NP_060322.1
• NCBI GenBank Nucleotide #: NM_017852
• UniProt Accession #: Q9NX02; Q53FL5; Q59G09; Q8IXT0; Q9BVN5; Q9H6G6; Q9HAV9; Q9NWK3; B4DZL7; I3L0G4
• Molecular Weight: 117,883 Da
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 2
• NCBI Official Symbol: NLRP2
• NCBI Official Synonym Symbols: NBS1; PAN1; NALP2; PYPAF2; CLR19.9
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 2; nucleotide-binding site protein 1; PYRIN-containing APAF1-like protein 2; NACHT, LRR and PYD containing protein 2; NACHT, leucine rich repeat and PYD containing 2; PYRIN domain and NACHT domain-containing p
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 2
• UniProt Gene Name: NLRP2
• UniProt Synonym Gene Names: NALP2; NBS1; PAN1; PYPAF2
• UniProt Entry Name: NALP2_HUMAN

NCBI Description

NALP proteins, such as NALP2, are characterized by an N-terminal pyrin (MIM 608107) domain (PYD) and are involved in the activation of caspase-1 (CASP1; MIM 147678) by Toll-like receptors (see TLR4; MIM 603030). They may also be involved in protein complexes that activate proinflammatory caspases (Tschopp et al., 2003 [PubMed 12563287]).[supplied by OMIM, Mar 2008]

Uniprot Description

NLRP2: Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Belongs to the NLRP family. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: cytoplasm

Molecular Function: protein binding; Pyrin domain binding; ATP binding

Biological Process: positive regulation of caspase activity; positive regulation of interleukin-1 beta secretion