Testing Data
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HinfI Restriction Enzyme
HinfI
Purity
Molecular Biology Grade
Synonyms
HinfI; HinfI restriction enzyme
Purity/Purification
Molecular Biology Grade
Methylation Effects
Dam: never overlaps-no effect
Dcm: never overlaps-no effect
CpG: may overlap-cleavage impaired
EcoKI: never overlaps-no effect
EcoBI: may overlap-blocked
Dcm: never overlaps-no effect
CpG: may overlap-cleavage impaired
EcoKI: never overlaps-no effect
EcoBI: may overlap-blocked
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded or double-stranded labeled oligonucleotide occured during incubation with 10 units of HinfI for 4 hours.
Lambda
148
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with HinfI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 1.3uM. More than 95% of these can be recut.
M13mp18/19
27
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion with HinfI (10u/ug lambda DNA x 16 hours).
pBR322
10
PhiX174
21
pTZ19R/U
9
pUC18/19
6
pUC57
5
Stability During Prolonged Incubation
A minimum of 0.1 units of HinfI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Preparation and Storage
-20 degree C
Testing Data
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Testing Data
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Product Categories/Family for HinfI restriction enzyme
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Product Notes
The HinfI (Catalog #AAA638389) is a Restriction Enzyme and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HinfI, Restriction Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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