Protein A/G Recombinant Protein
Protein A/G, Recombinant (Agarose)
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Molecular Biology GradePurified
Synonyms
Protein A/G; Recombinant (Agarose); Protein A/G recombinant protein
Purity/Purification
Molecular Biology Grade
Purified
Purified
Form/Format
Supplied as a 50% slurry in 0.01% thimerosal/H2O.
Applicable Applications for Protein A/G recombinant protein
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Purification of monoclonal and polyclonal antibodies. These conjugates may be used to track immunoglobulins in histochemical, western and ELISA applications.
Binding capacity
IgG binding is greater than 10mg of human IgG per 1ml of wet gel. High flow rate. Low falling off of rProtein A/G. pH stability 2-10.
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Protein A/G recombinant protein
Protein A/G Agarose is prepared by covalently coupling recombinant Protein A/G (contains five Ig-binding regions of protein A and three Ig-binding regions of protein G) to 6% cross-linked sepharose beads. The coupling was optimized to give a high binding capacity for IgG.
Protein G is a small globular cell surface protein produced by Streptococcus sp. It is composed of two or three nearly identical domains of 55 amino acids each. It binds to Fc fragment of IgG with high affinity.
Protein G will bind IgGs from many species including all human and mouse subclasses. It does not recognize IgM, IgA, IgE or serum albumin. Protein G binds IgG from rat,, but substantially more weakly than IgG from other species.
Recombinant Protein G is a highly stable surface receptor from Streptococcus sp. Lancefield Group G, produced in Escherichia coli, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987). Each protein G molecule can bind 2 molecules of IgG, allowing the formation of a precipitate.
Protein G may be coupled to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site.
Protein G is a small globular cell surface protein produced by Streptococcus sp. It is composed of two or three nearly identical domains of 55 amino acids each. It binds to Fc fragment of IgG with high affinity.
Protein G will bind IgGs from many species including all human and mouse subclasses. It does not recognize IgM, IgA, IgE or serum albumin. Protein G binds IgG from rat,, but substantially more weakly than IgG from other species.
Recombinant Protein G is a highly stable surface receptor from Streptococcus sp. Lancefield Group G, produced in Escherichia coli, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987). Each protein G molecule can bind 2 molecules of IgG, allowing the formation of a precipitate.
Protein G may be coupled to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site.
Product Categories/Family for Protein A/G recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
putative outer membrane protein A (3a;II*;G;d)
Similar Products
Product Notes
The Protein A/G (Catalog #AAA638174) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Protein A/G can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Purification of monoclonal and polyclonal antibodies. These conjugates may be used to track immunoglobulins in histochemical, western and ELISA applications. Researchers should empirically determine the suitability of the Protein A/G for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein A/G, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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