Frizzled 2, Fc Chimera Active Protein

Frizzled 2, Fc Chimera, Recombinant, Mouse (FZD2)

Cat. Num. AAA638109

• Purity: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Frizzled 2; Fc Chimera; Recombinant; Mouse (FZD2); Fc Chimera active protein

• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in 90mM Citrate, 0.26M sodium chloride, 0.25M Tris, pH 7.3.

• Activity: Measured by its ability to bind sFRP-2 in a functional ELISA. Immobilized mFrizzled-2/Fc at 2ug/ml (100ul/well) will bind rmsFRP-2 with a linear range of 1.6 -100ng/ml in a functional ELISA.
• Endotoxin Level: 1.0 EU per 1ug of the cytokine as determined by the LAL method.
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months from date of receipt at -20 degree C to -70 degree C. Upon reconstitution, this cytokine, in the presence of a carrier protein, can be stored under sterile conditions at 2 degree to 8 degree C for 2 weeks or at -20 degree C to -70 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for Frizzled 2, Fc Chimera active protein

Wnt signaling is involved in variety of developmental processes including cell fate determination, cell polarity, tissue patterning and control of cell proliferation. Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway. The founding member of this family was identified in Drosophila based on its role in tissue polarity in the adult cuticle and named for the disorganized appearance of bristle hairs on the mutant. Ten mammalian Frizzled genes have been identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail. One of the most conserved regions of the Frizzled proteins is the extracellular cysteine-rich domain (CRD) which spans approximately 120aa and contains 10 invariant cysteines (1). Mouse Frizzled-2 shows 100% amino acid identity to human and rat Frizzled-2 in the CRD region. Frizzled-2 expression is greater in embryonic than adult tissues, with heart, brain, lung, kidney and gut showing the highest levels (2). In addition, Frizzled-2 is expressed during migration and proliferation of neural crest cells that populate the heart and great arteries (3). However, Frizzled-2 may not be strictly involved in developmental processes,

Product Categories/Family for Frizzled 2, Fc Chimera active protein

Molecular Biology; MB-Wnt Proteins