Rabbit anti-Human GPC5 Polyclonal Antibody | anti-GPC5 antibody

GPC5 (Glypican 5, Glypican-5) (FITC)

Cat. Num. AAA6380168

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPC5; Polyclonal Antibody; GPC5 (Glypican 5; Glypican-5) (FITC); anti-GPC5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GPC5.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GPC5 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human GPC5, aa1-572 (NP_004457.1).
• Immunogen Sequence: MDAQTWPVGFRCLLLLALVGSARSEGVQTCEEVRKLFQWRLLGAVRGLPDSPRAGPDLQVCISKKPTCCTRKMEERYQIAARQDMQQFLQTSSSTLKFLISRNAAAFQETLETLIKQAENYTSILFCSTYRNMALEAAASVQEFFTDVGLYLFGADVNPEEFVNRFFDSLFPLVYNHLINPGVTDSSLEYSECIRMARRDVSPFGNIPQRVMGQMGRSLLPSRTFLQALNLGIEVINTTDYLHFSKECSRALLKMQYCPHCQGLALTKPCMGYCLNVMRGCLAHMAELNPHWHAYIRSLEELSDAMHGTYDIGHVLLNFHLLVNDAVLQAHLNGQKLLEQVNRICGRPVRTPTQSPRCSFDQSKEKHGMKTTTRNSEETLANRRKEFINSLRLYRSFYGGLADQLCANELAAADGLPCWNGEDIVKSYTQRVVGNGIKAQSGNPEVKVKGIDPVINQIIDKLKHVVQLLQGRSPKPDKWELLQLGSGGGMVEQVSGDCDDEDGCGGSGSGEVKRTLKITDWMPDDMNFSDVKQIHQTDTGSTLDTTGAGCAVATESMTFTLISVVMLLPGIW
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of GPC5 expression in transfected 293T cell line by GPC5 polyclonal antibody. Lane 1: GPC5 transfected lysate (63.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-GPC5 antibody

Cell surface proteoglycan that bears heparan sulfate.

Product Categories/Family for anti-GPC5 antibody

Antibodies; Proteoglycans

NCBI and Uniprot Product Information

• NCBI GI #: 4758464
• NCBI GeneID: 2262
• NCBI Accession #: NP_004457.1
• NCBI GenBank Nucleotide #: NM_004466.3
• UniProt Accession #: P78333; O60436; Q9BX27; B2R726
• Molecular Weight: 63,707 Da
• NCBI Official Full Name: glypican-5
• NCBI Official Synonym Full Names: glypican 5
• NCBI Official Symbol: GPC5
• NCBI Protein Information: glypican-5; bA93M14.1; glypican proteoglycan 5
• UniProt Protein Name: Glypican-5
• Protein Family: Glypican
• UniProt Gene Name: GPC5
• UniProt Entry Name: GPC5_HUMAN

NCBI Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]

Uniprot Description

GPC5: Cell surface proteoglycan that bears heparan sulfate. Belongs to the glypican family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Cell surface

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: lysosomal lumen; extracellular space; proteinaceous extracellular matrix; integral to plasma membrane; Golgi lumen; plasma membrane; extracellular region

Molecular Function: heparan sulfate proteoglycan binding

Biological Process: chondroitin sulfate metabolic process; phototransduction, visible light; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; retinoid metabolic process