Rabbit anti-Human GDF9 Polyclonal Antibody | anti-GDF9 antibody

GDF9 (Growth Differentiation Factor 9, Growth/Differentiation Factor 9, GDF-9) (HRP)

Cat. Num. AAA6379663

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GDF9; Polyclonal Antibody; GDF9 (Growth Differentiation Factor 9; Growth/Differentiation Factor 9; GDF-9) (HRP); anti-GDF9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GDF9.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GDF9 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human GDF9, aa1-454 (NP_005251.1).
• Immunogen Sequence: MARPNKFLLWFCCFAWLCFPISLGSQASGGEAQIAASAELESGAMPWSLLQHIDERDRAGLLPALFKVLSVGRGGSPRLQPDSRALHYMKKLYKTYATKEGIPKSNRSHLYNTVRLFTPCTRHKQAPGDQVTGILPSVELLFNLDRITTVEHLLKSVLLYNINNSVSFSSAVKCVCNLMIKEPKSSSRTLGRAPYSFTFNSQFEFGKKHKWIQIDVTSLLQPLVASNKRSIHMSINFTCMKDQLEHPSAQNGLFNMTLVSPSLILYLNDTSAQAYHSWYSLHYKRRPSQGPDQERSLSAYPVGEEAAEDGRSSHHRHRRGQETVSSELKKPLGPASFNLSEYFRQFLLPQNECELHDFRLSFSQLKWDNWIVAPHRYNPRYCKGDCPRAVGHRYGSPVHTMVQNIIYEKLDSSVPRPSCVPAKYSPLSVLTIEPDGSIAYKEYEDMIATKCTCR
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of GDF9 expression in transfected 293T cell line by GDF9 polyclonal antibody. Lane 1: GDF9 transfected lysate (51.4kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-GDF9 antibody

Growth differentiation factor 9 (GDF9) is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. BMP proteins are secreted growth factors that are characterized by seven conserved cysteine residues. In general, they are regulators of cell growth and differentiation in both embryonic and adult tissues. GDF9 can form non-covalent homodimers when expressed individually, while when GDF9 and BMP15 are co-expressed BMP-15/GDF-9 heterodimers are produced. GDF9 signaling might be transduced by binding to the bone morphogenetic protein receptor (BMPR) type II and one or more BMPR type I receptors.

Product Categories/Family for anti-GDF9 antibody

Antibodies; Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 4885261
• NCBI GeneID: 2661
• NCBI Accession #: NP_005251.1
• NCBI GenBank Nucleotide #: NM_005260
• UniProt Accession #: O60383; Q4VAW5
• Molecular Weight: 51,444 Da
• NCBI Official Full Name: growth/differentiation factor 9 preproprotein
• NCBI Official Synonym Full Names: growth differentiation factor 9
• NCBI Official Symbol: GDF9
• NCBI Protein Information: growth/differentiation factor 9; GDF-9
• UniProt Protein Name: Growth/differentiation factor 9
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF9
• UniProt Synonym Gene Names: GDF-9
• UniProt Entry Name: GDF9_HUMAN

NCBI Description

Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. Growth differentiation factor-9 (GDF9) is expressed in oocytes and is thought to be required for ovarian folliculogenesis. GDF9 is a member of the transforming growth factor-beta superfamily. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF9: Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells. Altered GDF9 function may be involved in ovarian disorders. Rare variants in GDF9 have been found in patients with premature ovarian failure and mothers of dizygotic twins. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; cytoplasm

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; BMP signaling pathway; oocyte growth; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; positive regulation of cell proliferation; female gamete generation; negative regulation of cell growth; cell development