Heme Oxygenase 2 Recombinant Protein | HMOX2 recombinant protein
Heme Oxygenase 2, Recombinant, Human (HO-2, HMOX2)
90% as determined by SDS-PAGE analysis.
90% as determined by SDS-PAGE analysis.
Dilution: Western Blot: We recommend that the material be diluted in 1X sample buffer (e.g. Laemmli, U.K., (1970) Nature 277: 680-685). On a 15-well minigel system, 50ng of protein per lane should be sufficient when used in a Western Blot. This protein mixture yields a band of 34.4kD. An ~70kD band is also observed, which may be aggregates of HO-2.
To date, three heme oxygenase isoforms HO-1, HO-2 and HO-3 have been identified. HO-1 or HSP32, a major heat shock/stress response protein is ubiquitous and its mRNA as well as its activity can be increased several-fold by heme, other metalloporphyrins, transition metals and stimuli that induce cellular stress. In contrast to HO-1, HO-2 is highly concentrated in neurons and testes (1). Multiple HO-2 transcripts which differ in size and use 3 different 5í untranslated regions (referred to as rHO-2, rHO-2-1 and rHO-2-2) and 2 poly(A) signals have been identified and a functional glucocorticoid response element (GRE) in the promoter region of rHO-2 has been characterized. In the adult rat testis, there is developmentally regulated expression of two transcripts for HO-2 of ~2.1kb and ~1.45kb which are not present in the brain, kidney, thymus, heart, spleen, liver or in prepubertal 14 day old rat testis. Both of these transcripts exclusively use rHO-2 and they contain all of the coding region exons present in the ~1.3kb and ~1.9kb transcripts which are common to all other organs including the adult and prepubertal rat testis. This data suggests that HO-2 levels in the testis are controlled by glucorticoids and that developmental and tissue-specific factor(s) determine generation of transcripts unique to the organ. The apparent exclusive use of rHO-2 by the mature testis suggests that HO-2 may play a role in male reproduction (2).
NCBI and Uniprot Product Information
NCBI Description
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Alternative splice variants encoding the same protein have been identified at this locus. [provided by RefSeq, Jul 2008]
Uniprot Description
HMOX2: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. Belongs to the heme oxygenase family.
Protein type: Oxidoreductase; EC 1.14.99.3; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: endoplasmic reticulum membrane; membrane; plasma membrane
Molecular Function: protein binding; metal ion binding; heme oxygenase (decyclizing) activity
Biological Process: heme catabolic process; cellular iron ion homeostasis; porphyrin metabolic process; response to hypoxia; heme oxidation; transmembrane transport
Research Articles on HMOX2
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Product Notes
The HMOX2 hmox2 (Catalog #AAA637916) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Heme Oxygenase 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry (1:2,000). Dilution: Western Blot: We recommend that the material be diluted in 1X sample buffer (e.g. Laemmli, U.K., (1970) Nature 277: 680-685). On a 15-well minigel system, 50ng of protein per lane should be sufficient when used in a Western Blot. This protein mixture yields a band of 34.4kD. An ~70kD band is also observed, which may be aggregates of HO-2. Researchers should empirically determine the suitability of the HMOX2 hmox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heme Oxygenase 2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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