Urokinase Plasminogen Activator Receptor 1 Recombinant Active Protein
Urokinase Plasminogen Activator Receptor 1 Recombinant, Human (uPAR-1/Fc)
Applications
ELISA
Purity
Purified90%, as determined by SDS-PAGE and visualized by silver stain.
Synonyms
Urokinase Plasminogen Activator Receptor 1 Recombinant; Human (uPAR-1/Fc); Urokinase Plasminogen Activator Receptor 1 Recombinant active protein
Purity/Purification
Purified
90%, as determined by SDS-PAGE and visualized by silver stain.
90%, as determined by SDS-PAGE and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder in PBS. Reconstitite with PBS, 0.1% BSA or HSA, to a concentration of 0.05mg/ml
Applicable Applications for Urokinase Plasminogen Activator Receptor 1 Recombinant active protein
ELISA (EL/EIA)
Activity
Immobilized mouse uPAR/Fc at 5ug/ml (100uL/well) can bind rhuPA with a linear range of 3-200ng/ml in a functional ELISA assay.
Endotoxin Level
1.0 EU/ug of the cytokine as determined by the LAL method.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Related Product Information for Urokinase Plasminogen Activator Receptor 1 Recombinant active protein
Mouse uPAR-1/Fc Chimera NS0-derived recombinant mouse uPAR-1. Corresponds to a region between aa1 and 297.
The urokinase-type plasminogen activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity.
The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. Mouse uPAR-1/Fc cDNA encodes a 327 amino acid residue precursor protein with a 23 aa residue signal peptide, seven potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% amino acid sequence identity and the receptor-ligand interaction is highly species-specific.
The urokinase-type plasminogen activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity.
The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. Mouse uPAR-1/Fc cDNA encodes a 327 amino acid residue precursor protein with a 23 aa residue signal peptide, seven potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% amino acid sequence identity and the receptor-ligand interaction is highly species-specific.
Product Categories/Family for Urokinase Plasminogen Activator Receptor 1 Recombinant active protein
Similar Products
Product Notes
The Urokinase Plasminogen Activator Receptor 1 Recombinant (Catalog #AAA637882) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Urokinase Plasminogen Activator Receptor 1 Recombinant can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Researchers should empirically determine the suitability of the Urokinase Plasminogen Activator Receptor 1 Recombinant for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Urokinase Plasminogen Activator Receptor 1 Recombinant, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
