Rabbit anti-Human, Mouse DGCR8 Polyclonal Antibody | anti-DGCR8 antibody

DGCR8 (Microprocessor Complex Subunit DGCR8, DiGeorge Syndrome Critical Region 8, C22orf12, DGCRK6, LP4941) (Biotin)

Cat. Num. AAA6376064

• Gene Names: DGCR8; Gy1; pasha; DGCRK6; C22orf12
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DGCR8; Polyclonal Antibody; DGCR8 (Microprocessor Complex Subunit DGCR8; DiGeorge Syndrome Critical Region 8; C22orf12; DGCRK6; LP4941) (Biotin); anti-DGCR8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DGCR8. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DGCR8 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human DGCR8, aa1-773 (NP_073557.3).
• Immunogen Sequence: METDESPSPLPCGPAGEAVMESRARPFQALPREQSPPPPLQTSSGAEVMDVGSGGDGQSELPAEDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGAERDVRAECGLLLSPVSGDVHACPFGGSVGDGVGIGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGAGGFTAKAIVQRDRVDEEALNFPYEDDFDNDVDALLEEGLCAPKKRRTEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQSSDLTPSGDVSPVKPLSRSAELEFPLDEPDSMGADPGPPDEKDPLGAEAAPGALGQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DGCR8 rabbit polyclonal antibody. Western Blot analysis of DGCR8 expression in mouse lung.)

Western Blot (WB)

(Western Blot analysis of DGCR8 expression in transfected 293T cell line by DGCR8 polyclonal antibody. Lane 1: DGCR8 transfected lysate (86kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-DGCR8 antibody

Component of the microprocessor complex that acts as a RNA-and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.

Product Categories/Family for anti-DGCR8 antibody

Antibodies; Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 298358603
• NCBI GeneID: 54487
• NCBI Accession #: NP_073557.3
• NCBI GenBank Nucleotide #: NM_022720.5
• Molecular Weight: 82,750 Da
• NCBI Official Full Name: Homo sapiens DGCR8 microprocessor complex subunit (DGCR8), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: DGCR8 microprocessor complex subunit
• NCBI Official Symbol: DGCR8
• NCBI Official Synonym Symbols: Gy1; pasha; DGCRK6; C22orf12
• NCBI Protein Information: microprocessor complex subunit DGCR8; DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8
• Protein Family: Microprocessor complex

NCBI Description

This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]