GST-mu Active Protein
GST-mu, Recombinant, Human (Glutathione S Transferase mu, GSTmu)
Applications
ELISA, Western Blot
Purity
Highly Purified (>95%)
Synonyms
GST-mu; Recombinant; Human (Glutathione S Transferase mu; GSTmu); GST-mu active protein
Host
Human GST-my/GST M1-1, purified, expressed in E.coli.
Purity/Purification
Highly Purified (>95%)
Form/Format
Supplied as a liquid in 50mM Tris, pH7.5, 50mM sodium chloride; 1mM DTT, 1mM EDTA, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for GST-mu active protein
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in biological activity determination, ELISA, and for standards or for positive control for Western Blot. Other applications not tested.
Recommended Dilutions: Optimal dilutions to be determined by the researcher.
Recommended Dilutions: Optimal dilutions to be determined by the researcher.
Specific Activity
~50units/mg
Under native conditions, GST-mu is homodimeric protein of ~50kd and under reducing conditions ~25kD.
Under native conditions, GST-mu is homodimeric protein of ~50kd and under reducing conditions ~25kD.
Unit Definition
One unit will catalyze the conjugation of 1 micromole of 1-chloro-2-4-dinitrobenzene and reduced glutathione per min at pH 6.5 at 25 degree C.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for GST-mu active protein
GST's are responsible for the metabolism of a broad range of xenobiotics and carcinogens. The enzyme catalyzes the reaction of glutathione with a wide variety of organic compounds to form thioethers, a reaction that is sometimes a first step in a detoxification process leading to mercapturic acid formation. Based on aa sequence similarities and antibody cross-reactivities, the mammalian cytosolic GSTs are divided into the following classes: alpha, mu, kappa, theta, pi, zeta, omega and sigma. GSTs exists as homodimers of ~25kD. GST A1-1 is mainly found in kidney, intestine, lung, and liver. GST M1-1 is restricted to liver and few other tissues. GST P1-1 has a widespread distribution in most tumor cells and tissues but it is absent in liver. The GST M1-1 is the type b allelic variant and corresponds to human GST Y.
The GST M1 along with GST M2-GST M5 are mapped to Chrm 1, There is a close physical proximity between GSTM1 and GSTM2 loci, which shares 99% nucleotide sequence identity over 460 nucleotides of 3-prime untranslated mRNA. The 181aa long GST M1 is mainly expressed in liver, brain, testis, kidney and lung and show enzyme activity towards CDNB; AFB1 - epoxide; trans-4-phenyl-3buten-2-one.
The GST M1 along with GST M2-GST M5 are mapped to Chrm 1, There is a close physical proximity between GSTM1 and GSTM2 loci, which shares 99% nucleotide sequence identity over 460 nucleotides of 3-prime untranslated mRNA. The 181aa long GST M1 is mainly expressed in liver, brain, testis, kidney and lung and show enzyme activity towards CDNB; AFB1 - epoxide; trans-4-phenyl-3buten-2-one.
Product Categories/Family for GST-mu active protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The GST-mu (Catalog #AAA637319) is an Active Protein produced from Human GST-my/GST M1-1, purified, expressed in E.coli. and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GST-mu can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in biological activity determination, ELISA, and for standards or for positive control for Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the GST-mu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GST-mu, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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