ERBB4 active protein

ErbB4, Recombinant, Human (c-erbB4, HER4)

Cat. Num. AAA637268

• Gene Names: ERBB4; HER4; ALS19; p180erbB4
• Purity: Highly Purified; 0.9
• Synonyms: ErbB4; Recombinant; Human (c-erbB4; HER4); ERBB4 active protein

• Purity/Purification: Highly Purified; 0.9
• Form/Format: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween-20, 3mM DTT, 50% glycerol.

• Application Notes: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Specific Activity: 3.29 pmol/min/ug
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Testing Data

(specific activity)

SDS-Page

(10% SDS-PAGE staining of E3451-40H. Lane 1: 10ug E3451-40H. Lane 2: Protein Marker. MW: 59kD.)

Related Product Information for ERBB4 active protein

Human HER4 kinase (ERBB4) (GenBank Accession No. NM_005235), amino acids 708-993.

Product Categories/Family for ERBB4 active protein

Molecular Biology; MB-Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 219520060
• NCBI GeneID: 2066
• UniProt Accession #: Q15303; Q2M1W1; Q59EW4; B7ZLD7; B7ZLE2; B7ZLE3
• Molecular Weight: 59.5kD
• NCBI Official Full Name: ERBB4 protein
• NCBI Official Synonym Full Names: v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4
• NCBI Official Symbol: ERBB4
• NCBI Official Synonym Symbols: HER4; ALS19; p180erbB4
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-4; proto-oncogene-like protein c-ErbB-4; tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-4
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: ERBB4
• UniProt Synonym Gene Names: HER4; 4ICD; E4ICD
• UniProt Entry Name: ERBB4_HUMAN

NCBI Description

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HER4: a receptor tyrosine kinase of the EGFR family. Specifically binds and is activated by neuregulins, NRG- 2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Heterodimerizes and signals with other EGF receptors. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with WWOX. May act as a tumor suppressor: overexpressed in head and neck cancer , but downregulated in renal cancer, papillary carcinoma, high-grade gliomas and invasive breast cancer. 3 alternatively-spliced isoforms of the human protein have been reported.

Protein type: Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; TK group; EGFR family

Chromosomal Location of Human Ortholog: 2q33.3-q34

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; basolateral plasma membrane; extracellular region; plasma membrane; integral to membrane; nucleus; cytosol; receptor complex

Molecular Function: protein binding; protein homodimerization activity; protein-tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; epidermal growth factor receptor binding

Biological Process: lactation; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; heart development; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; embryonic pattern specification; signal transduction; olfactory bulb interneuron differentiation; regulation of cell migration; negative regulation of cell proliferation; positive regulation of cell proliferation; neural crest cell migration; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; cell migration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; cell fate commitment; transcription, DNA-dependent; positive regulation of phosphoinositide 3-kinase activity; cell proliferation; central nervous system morphogenesis; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response; mitochondrial fragmentation during apoptosis; positive regulation of protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Amyotrophic Lateral Sclerosis 19