CD200R1, Fc Chimera Active Protein
CD200R1, Fc Chimera, Recombinant, Mouse (OX2R, OX2 Receptor)
Purity
85%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Synonyms
CD200R1; Fc Chimera; Recombinant; Mouse (OX2R; OX2 Receptor); Fc Chimera active protein
Host
Recombinant Mouse
Purity/Purification
85%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.
Concentration
~0.1 mg/mL (after reconstitution) (varies by lot)
Activity
Measured by its ability to bind in a functional ELISA.
Endotoxin Level
<0.01 EU/ug
Molecular Mass
Calculated molecular mass of 49.7kD. As a result of glycosylation, the recombinant monomer migrates as an ~ 90-100kD protein in SDS-PAGE under reducing conditions.
Preparation and Storage
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for CD200R1, Fc Chimera active protein
CD200 R1, also known as OX-2 receptor, is a 90kD, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity.1-3 The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a 25 aa signal sequence, a 213 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain.4 Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, 5-7 while its ligand, CD200, is widely distributed.8 Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders.9 Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains.10 The CD200 R-like molecules may interact differently with CD200.11, 12 The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals.13-15 CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12.4, 16
A DNA sequence encoding the extracellular domain of mouse CD200 R1 (Met 1-Pro 236; Accession # BAC40777) was fused to the carboxy-terminal Fc region of human IgG1 via a linker peptide. The chimeric protein was expressed in a mouse myeloma cell line, NS0
A DNA sequence encoding the extracellular domain of mouse CD200 R1 (Met 1-Pro 236; Accession # BAC40777) was fused to the carboxy-terminal Fc region of human IgG1 via a linker peptide. The chimeric protein was expressed in a mouse myeloma cell line, NS0
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Product Notes
The CD200R1, Fc Chimera (Catalog #AAA637024) is an Active Protein produced from Recombinant Mouse and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD200R1, Fc Chimera, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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