SDS-Page
(SDS-PAGE/Coomassie staining using 1ug of G8965-10E. Arrow indicates GFP (~28kD).)
Green Fluorescent Protein Recombinant Protein
Green Fluorescent Protein, Recombinant (GFP)
Applications
Western Blot
Purity
PurifiedPurified using Ni-NTA agarose (~70%).
Synonyms
Green Fluorescent Protein; Recombinant (GFP); Green Fluorescent Protein recombinant protein
Host
E Coli
Purity/Purification
Purified
Purified using Ni-NTA agarose (~70%).
Purified using Ni-NTA agarose (~70%).
Form/Format
Supplied as a liquid in PBS, 20% glycerol.
Applicable Applications for Green Fluorescent Protein recombinant protein
Western Blot (WB)
Application Notes
Suitable for use as a standard for protein gels and Western Blots (~28kD). Also suitable for calibration of uorometers and microinjection into cells and tissues.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(SDS-PAGE/Coomassie staining using 1ug of G8965-10E. Arrow indicates GFP (~28kD).)
SDS-Page
(SDS-PAGE/Coomassie staining using 1ug of G8965-10E. Arrow indicates GFP (~28kD).)
Related Product Information for Green Fluorescent Protein recombinant protein
Full-length Green Fluorescent Protein with N-terminal HIS-tag (6X), recombinant, expressed in E. coli.
GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications.
GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications.
Product Categories/Family for Green Fluorescent Protein recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
~28kD
NCBI Official Full Name
green fluorescent protein
Protein Family
Similar Products
Product Notes
The Green Fluorescent Protein (Catalog #AAA636982) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Green Fluorescent Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use as a standard for protein gels and Western Blots (~28kD). Also suitable for calibration of uorometers and microinjection into cells and tissues. Researchers should empirically determine the suitability of the Green Fluorescent Protein for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Green Fluorescent Protein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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