Angiopoietin 1 Active Protein | ANGPT1 active protein

Angiopoietin 1, Recombinant, Human (Agpt, ang1, Ang1, Ang-1, ANG-1, Angiopoietin-1)

Cat. Num. AAA636948

• Gene Names: ANGPT1; AGP1; AGPT; ANG1
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: 90%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Angiopoietin 1; Recombinant; Human (Agpt; ang1; Ang1; Ang-1; ANG-1; Angiopoietin-1); ANGPT1 active protein

• Reactivity: Mouse
• Purity/Purification: 90%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder from 50mM Tris-citrate, 500mM sodium chloride, pH 6.5.

• Applicable Applications for ANGPT1 active protein: ELISA (EL/EIA), Western Blot (WB)
• Biological Activity: Measured by its ability to inhibit serum deprivation induced apoptosis in human umbilical vein endothelial cells (HUVEC) (6). The ED50 for this effect is typically 10-40ng/ml in the presence of 5ug/ml of a cross-linking antibody (mouse anti-6X histidine).
• Endotoxin Level: 1EU/ug (LAL)
• Reconstitution: Reconstitute with sterile PBS to 100ug/ml.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for ANGPT1 active protein

Tie-1 (human 1138 aa; mouse 1134 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Murine and human Ang-1 are 498aa secreted angiogenic proteins. It is only 60% homologous with Ang-2. Ang-1 is the natural ligand for Tie-2 and is primarily expressed in developing endothelial cells.

Product Categories/Family for ANGPT1 active protein

Molecular Biology; MB-Angiopoietin

NCBI and Uniprot Product Information

• NCBI GI #: 22203641
• NCBI GeneID: 284
• UniProt Accession #: Q15389; Q5HYA0
• Molecular Weight: Based on N-terminal sequencing, the recombinant human angiopoietin-1 protein starts at Ser 20 and has a calculated molecular mass of 56kD. As a result of glycosylation, the recombinant protein migrates as an approximately 70kD band in SDS-PAGE under reduc
• NCBI Official Full Name: angiopoietin 1
• NCBI Official Synonym Full Names: angiopoietin 1
• NCBI Official Symbol: ANGPT1
• NCBI Official Synonym Symbols: AGP1; AGPT; ANG1
• NCBI Protein Information: angiopoietin-1; ANG-1
• UniProt Protein Name: Angiopoietin-1
• Protein Family: Angiopoietin
• UniProt Gene Name: ANGPT1
• UniProt Synonym Gene Names: KIAA0003; ANG-1
• UniProt Entry Name: ANGP1_HUMAN

NCBI Description

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2010]

Uniprot Description

ANGPT1: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: extracellular space; microvillus; plasma membrane; extracellular region; lipid raft

Molecular Function: receptor tyrosine kinase binding

Biological Process: positive regulation of cell adhesion; transmembrane receptor protein tyrosine kinase activation (dimerization); positive regulation of receptor internalization; negative regulation of protein import into nucleus; negative regulation of cell adhesion; positive chemotaxis; negative regulation of protein amino acid phosphorylation; hemopoiesis; negative regulation of neuron apoptosis; regulation of I-kappaB kinase/NF-kappaB cascade; cell differentiation; regulation of endothelial cell proliferation; Tie receptor signaling pathway; in utero embryonic development; positive regulation of blood vessel endothelial cell migration; positive regulation of peptidyl-serine phosphorylation; cell-substrate adhesion; negative regulation of cytokine secretion during immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; heparin biosynthetic process; positive regulation of protein ubiquitination; regulation of protein binding; negative regulation of vascular permeability; regulation of tumor necrosis factor production; sprouting angiogenesis; regulation of satellite cell proliferation; blood coagulation; leukocyte migration; negative regulation of apoptosis