Hepsin Active Protein | HPN active protein

Hepsin, Recombinant, Human (TMPRSS1)

Cat. Num. AAA636703

• Gene Names: HPN; TMPRSS1
• Purity: Purified; Purified 95% (SDS-PAGE). Endotoxin: 1.0 EU/1ug (LAL).
• Synonyms: Hepsin; Recombinant; Human (TMPRSS1); HPN active protein

• Specificity: Recognizes human Hepsin.
• Purity/Purification: Purified; Purified 95% (SDS-PAGE). Endotoxin: 1.0 EU/1ug (LAL).
• Form/Format: Supplied as a 0.2um sterile-filtered solution in 50mM sodium acetate, 1M sodium chloride, pH 5.

• Activity: Measured under the described conditions, is > 20,000 pmole/min/ug.
• Preparation and Storage: 12 months from date of receipt, 20 to 70 degree C as supplied.; 1 month, 2 to 8 degree C under sterile conditions after reconstitution.; 3 months, 20 to 70 degree C under sterile conditions after reconstitution.; Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Related Product Information for HPN active protein

Hepsin, also known as TMPRSS1, is a Type II membrane protein with an extracellular serine protease domain (1). It is most highly expressed in liver, but is also present in many other tissues, notably lung, kidney, and skeletal muscle (2). A soluble form of Hepsin lacking the transmembrane domain has been identified (3). Hepsin is capable of activating Factor VII, and may initiate blood coagulation at the cell surface (4). Hepsin is overexpressed in various human tumors, including prostate (5), and is considered to be a biomarker for prostate cancer (6). rhHepsin was expressed as a secreted, soluble protein lacking its cytosolic and transmembrane domains. The D161E and R162K mutations were introduced into the prosequence to improve expression of the rhHepsin.

Product Categories/Family for HPN active protein

Molecular Biology; MB-Membrane Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 306886
• NCBI GeneID: 3249
• UniProt Accession #: P05981; B2RDS4
• Molecular Weight: 42kD
• NCBI Official Full Name: hepsin (serine protease)
• NCBI Official Synonym Full Names: hepsin
• NCBI Official Symbol: HPN
• NCBI Official Synonym Symbols: TMPRSS1
• NCBI Protein Information: serine protease hepsin; transmembrane protease serine 1
• UniProt Protein Name: Serine protease hepsin
• Protein Family: Histidine-rich metal-binding polypeptide
• UniProt Gene Name: HPN
• UniProt Synonym Gene Names: TMPRSS1
• UniProt Entry Name: HEPS_HUMAN

NCBI Description

This gene encodes a type II transmembrane serine protease that may be involved in diverse cellular functions, including blood coagulation and the maintenance of cell morphology. Expression of the encoded protein is associated with the growth and progression of cancers, particularly prostate cancer. The protein is cleaved into a catalytic serine protease chain and a non-catalytic scavenger receptor cysteine-rich chain, which associate via a single disulfide bond. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

HPN: Plays an essential role in cell growth and maintenance of cell morphology. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.106; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; cell surface; cell soma; integral to plasma membrane; integral to membrane; plasma membrane; intercellular junction

Molecular Function: peptidase activity; calcium-activated potassium channel activity; protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: regulation of cell shape; cholesterol homeostasis; detection of mechanical stimulus involved in sensory perception of sound; proteolysis; positive regulation of cell growth; negative regulation of epithelial cell proliferation; negative regulation of apoptosis