Activin A Receptor Type IB Active Protein | ACVR1B active protein

Activin A Receptor Type IB, Recombinant, Mouse (RIB/Fc Chimera, Activin Receptor-like Kinase 4, Activin Receptor Type 1B Precursor, ACTRIB, ACTR-IB, ACVRLK4, ALK4, ALK-4, Serine/threonine-protein Kinase Receptor R2, SKR2)

Cat. Num. AAA636609

• Gene Names: ACVR1B; ALK4; SKR2; ACTRIB; ACVRLK4
• Reactivity: Mouse
• Purity: Purified; ~90%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Activin A Receptor Type IB; Recombinant; Mouse (RIB/Fc Chimera; Activin Receptor-like Kinase 4; Activin Receptor Type 1B Precursor; ACTRIB; ACTR-IB; ACVRLK4; ALK4; ALK-4; Serine/threonine-protein Kinase Receptor R2; SKR2); ACVR1B active protein

• Reactivity: Mouse
• Specificity: Recognizes human Activin A Receptor Type IB.
• Purity/Purification: Purified; ~90%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS. Reconstitute with PBS to a concentration of 100ug/ml.

• Immunogen: NS0-derived recombinant mature mouse Activin RIB/Fc.
• Biological Activity: Measured by its ability to bind rmCripto. Immobilized rmActivin RIB/Fc at 1ug/ml (100uL/well) will bind rmCripto with a linear range of 0.8-100ng/mL.
• Endotoxin Level: 1.0 EU per 1ug of protein as determined by the LAL method.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for ACVR1B active protein

Activin RIB, also known as ALK4, is a glycosylated 58kD type I transmembrane receptor that belongs to the superfamily of TGF-b serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with, and phosphorylates, the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature mouse Activin RIB consists of a 103 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, mouse Activin RIB shares 93% and 98% aa sequence identity with human and rat Activin RIB, respectively. It shares 23%-38% aa sequence identity with other mouse type I receptors Activin RIA, BMPR-IA, BMPR-IB, and TGF-b RI. Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (5-7). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (8). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation, apoptosis, and tumorigenesis (1, 9, 10).

A DNA sequence encoding the extracellular domain of mouse Activin RIB was fused to the Fc region of human IgG1 via a linker peptide. The chimeric protein was expressed in a mouse myeloma cell line, NS0.
The recombinant mature mouse Activin RIB/Fc chimera is a disulfide-linked homodimeric protein. Based on N-terminal amino acid sequencing, the mature recombinant protein starts at Leu 32. The recombinant mouse Activin RIB/Fc monomer has a calculated molecular mass of 37.2kD. As a result of glycosylation, the recombinant monomer migrates as an approximately 44kD protein in SDS-PAGE under reducing conditions.

Product Categories/Family for ACVR1B active protein

Molecular Biology; MB-Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 26252117
• NCBI GeneID: 91
• UniProt Accession #: P36896; Q15479; Q15480; Q15481; Q15482; B7Z5L8; B7Z5W5
• Molecular Weight: 56,807 Da
• NCBI Official Full Name: Activin A receptor, type IB
• NCBI Official Synonym Full Names: activin A receptor, type IB
• NCBI Official Symbol: ACVR1B
• NCBI Official Synonym Symbols: ALK4; SKR2; ACTRIB; ACVRLK4
• NCBI Protein Information: activin receptor type-1B; activin receptor-like kinase 4; activin A receptor, type II-like kinase 4; serine/threonine-protein kinase receptor R2
• UniProt Protein Name: Activin receptor type-1B
• Protein Family: Activin receptor
• UniProt Gene Name: ACVR1B
• UniProt Synonym Gene Names: ACVRLK4; ALK4; ACTR-IB; ALK-4; SKR2
• UniProt Entry Name: ACV1B_HUMAN

NCBI Description

This gene encodes an activin A type IB receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I and two type II receptors. This protein is a type I receptor which is essential for signaling. Mutations in this gene are associated with pituitary tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]

Uniprot Description

ALK4: Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine- threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C- terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2. ACVRIB is abundantly expressed in systemic sclerosis patient fibroblasts and production of collagen is also induced by activin-A/INHBA. This suggests that the activin/ACRV1B signaling mechanism is involved in systemic sclerosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Kinase, protein; Protein kinase, TKL; EC 2.7.11.30; TKL group; STKR family; Type1 subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; activin receptor complex; receptor complex

Molecular Function: activin receptor activity; metal ion binding; transmembrane receptor protein serine/threonine kinase activity; transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; activin receptor activity, type I; ubiquitin protein ligase binding; growth factor binding; activin binding; SMAD binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: development of primary female sexual characteristics; positive regulation of activin receptor signaling pathway; central nervous system development; regulation of transcription, DNA-dependent; hair follicle development; in utero embryonic development; positive regulation of erythrocyte differentiation; protein amino acid autophosphorylation; transmembrane receptor protein serine/threonine kinase signaling pathway; peptidyl-threonine phosphorylation; activin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; signal transduction; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle