BTLA, Fc Chimera Active Protein
BTLA, Fc Chimera, Recombinant, Mouse (B- and T-Lymphocyte Attenuator, CD272)
Purity
Highly Purified90%, as determined by SDS-PAGE and visualized by silver stain.
Synonyms
BTLA; Fc Chimera; Recombinant; Mouse (B- and T-Lymphocyte Attenuator; CD272); Fc Chimera active protein
Purity/Purification
Highly Purified
90%, as determined by SDS-PAGE and visualized by silver stain.
90%, as determined by SDS-PAGE and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder in PBS, 5% trehalose.
Activity
Measured by its ability to bind immobilized rhHVEM/Fc (Gonzalez, LC, et. al., 2005, PNAS 102:1116).
Reconstitution
It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
Storage:
Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Storage:
Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Preparation and Storage
-20 degree C
Related Product Information for BTLA, Fc Chimera active protein
B- and T-lymphocyte attenuator (BTLA; CD272) is a 70kD, Ig-superfamily, type I transmembrane glycoprotein that is structurally similar to the CD28 family of T cell costimulatory or coinhibitory molecules. (1-3) Unlike CD28 family members, however, the BTLA extracellular Ig domain is an I-type rather than V-type domain, and BTLA does not form homodimers. (4) BTLA also differs from CD28 family members through the interaction of its Ig domain with the TNF superfamily member HVEM (herpesvirus entry mediator; TNFSF14) rather than with B7 family ligands. (5) BTLA is a coinhibitory molecule expressed on T cells, B cells and, depending on the mouse strain, macrophages, dendritic and NK cells. (6) Expression is low in naïve T cells and increased during antigen-specific induction of anergy. In B cells, BTLA is highest when cells are mature and naïve. (6) BTLA apparently limits T cell numbers, since deletion of BTLA results in overproduction of T cells, especially CD8+ memory T cells that are hyper-responsive to TCR crosslinking. (7) The 305 amino acid (aa) BTLA contains a 29 aa signal sequence, a 153 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 102 aa cytoplasmic domain. There are two ITIM motifs and three Tyr phosphorylation sites in the cytoplasmic tail that mediate inhibitory signaling. (8, 9) The binding of the BTLA to HVEM does not preclude additional binding of a mammalian stimulatory HVEM ligand, either LIGHT or lymphotoxin- to the complex. (4) At least three alleles varying by up to ten extracellular amino acids occur in different mouse strains. (6) The ECD of C57BL/6 BTLA shows 51%, 77% and 40% aa identity to that of human, rat and dog BTLA, respectively. A splice variant lacking the Ig domain, termed BTLAs, has been reported. (3)
Product Categories/Family for BTLA, Fc Chimera active protein
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Product Notes
The BTLA, Fc Chimera (Catalog #AAA636564) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BTLA, Fc Chimera, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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