Integrin alphaL, beta2 Active Protein | Itgb2 active protein

Integrin alphaL, beta2, Recombinant, Human (LFA-1, CD11a/CD18)

Cat. Num. AAA636537

• Gene Names: Itgb2; 2E6; LAD; Cd18; Lfa1; MF17; LCAMB; AI528527
• Purity: Highly Purified; ~95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1EU/ug (LAL).
• Synonyms: Integrin alphaL; beta2; Recombinant; Human (LFA-1; CD11a/CD18); Itgb2 active protein

• Purity/Purification: Highly Purified; ~95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1EU/ug (LAL).
• Form/Format: Supplied as a lyophilized powder from 50mM Tris, 0.15M sodium chloride, 2mM magnesium chloride, pH 7.4. Reconstitute with 500ul sterile PBS.

• Application Notes: SDS-PAGE: ~160kD and 100kD under reducing conditions. Predicted MW: 121.7kD (Integrin aL), 79kD (Integrin b2)
• Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of CHO cells transfected with human ICAM-1. When 5x10e4 cells/well are added to Integrin aLb2-coated plates (5ug/ml, 100ul/well), 60-80% of the cells will adhere after 1 hour at 37 degree C in the presence of 1mM MnCl2.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Itgb2 active protein

Integrin alphaLbeta2 is one of three beta2 integrin adhesion proteins. The non-covalent heterodimer of 180kD aL/CD11a and 95kD b2/CD18 integrin subunits is expressed on virtually all leukocytes. The ligand binding site of LFA-1 is in the N-terminal head region, formed by an interaction of the vWFA (I, I-like) domains from each subunit, and the aL beta­propeller structure. The aL subunit contains domains termed thigh, calf-1 and calf-2, while the b2 subunit contains a PSI region and four cysteine-rich I-EGF folds. Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Upon activation by "inside-out" signaling, clustering, or Mg2+ or Mn2+ binding to metal ion-dependent adhesion sites within the vWFA domains, the molecule unfolds from its inactive, "closed" conformation to expose ligand binding sites. Active aLb2 binds ICAM-1/CD54, ICAM-2, ICAM-3 and JAM-A. The adhesion stabilizes interactions between T cells and antigen-presenting cells, decreases the T cell activation threshold and facilitates leukocyte transendothelial migration to sites of inflammation. A constitutively active construct severely impairs immune responses, demonstrating that both activation and de-activation are important. Mutations of b2, especially in the vWFA domain, cause leukocyte adhesion deficiency (LAD-1) and susceptibility to bacterial infections. The 1088aa human aL/CD11b is 73-74% aa identical to mouse and rat, and 79-80% aa identical to bovine, porcine, ovine, goat and canine. The 678aa human b2/CD18 shares 81-83% aa sequence identity with mouse, rat, bovine, canine, goat, ovine and porcine b2 ECD. A second human aL isoform has 53 aa inserted after aa 954 in the ECD. The 678 aa human b 2/CD18 ECD shares 81-83% aa sequence identity with mouse, rat, bovine, canine, goat, ovine and porcine b2 ECD.

Product Categories/Family for Itgb2 active protein

Molecular Biology; MB-Integrins

NCBI and Uniprot Product Information

• NCBI GI #: 111607447
• NCBI GeneID: 16414
• NCBI Accession #: NP_032430.2
• NCBI GenBank Nucleotide #: NM_008404.4
• UniProt Accession #: P11835; Q64482
• Molecular Weight: Based on N-terminal sequencing, the recombinant human Integrin aL subunit starts at Tyr 26 and has a calculated molecular mass of approximately 121.7kD. The N-terminal sequence of the b2 subunit was blocked, suggesting that the b2 subunit may start at the
• NCBI Official Full Name: integrin beta-2
• NCBI Official Synonym Full Names: integrin beta 2
• NCBI Official Symbol: Itgb2
• NCBI Official Synonym Symbols: 2E6; LAD; Cd18; Lfa1; MF17; LCAMB; AI528527
• NCBI Protein Information: integrin beta-2; Mac-1 beta; macrophage antigen-1 beta; complement receptor C3 subunit beta; lymphocyte function associated antigen 1; cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta
• UniProt Protein Name: Integrin beta-2
• Protein Family: Integrin
• UniProt Gene Name: Itgb2
• UniProt Entry Name: ITB2_MOUSE

Uniprot Description

ITGB2: the integrin beta 2 subunit. Can combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Participates in cell adhesion as well as cell-surface mediated signaling. Defects are the cause of leukocyte adhesion deficiency type I (LAD1).

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis; Cell adhesion

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; integrin complex; vesicle; receptor complex; lipid raft

Molecular Function: protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; receptor activity; ICAM-3 receptor activity; cell adhesion molecule binding; glycoprotein binding; protein kinase binding

Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; positive regulation of nitric oxide biosynthetic process; multicellular organismal development; cell-matrix adhesion; activated T cell proliferation; natural killer cell activation; signal transduction; receptor clustering; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; heterotypic cell-cell adhesion; cell-cell adhesion; positive regulation of angiogenesis; leukocyte adhesion; cellular extravasation; endothelial cell migration; cell adhesion; regulation of peptidyl-tyrosine phosphorylation