Rabbit anti-Human XPO5 Polyclonal Antibody | anti-XPO5 antibody

XPO5, CT (XPO5, KIAA1291, RANBP21, Exportin-5, Ran-binding protein 21) (APC)

Cat. Num. AAA6364029

• Gene Names: XPO5; exp5
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: XPO5; Polyclonal Antibody; CT (XPO5; KIAA1291; RANBP21; Exportin-5; Ran-binding protein 21) (APC); Ran-binding protein 21; anti-XPO5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-XPO5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: XPO5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1122-1152 amino acids from the C-terminal region of human XPO5.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(XPO5 Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the XPO5 antibody detected the XPO5 protein (arrow).)

Related Product Information for anti-XPO5 antibody

Exportin-5 belongs to a large family of karyopherins (see MIM 602738) that mediate the transport of proteins and other cargo between the nuclear and cytoplasmic compartments.[supplied by OMIM].

Product Categories/Family for anti-XPO5 antibody

Antibodies; Binding Proteins

References

Melo, S.A., et al. Cancer Cell 18(4):303-315(2010). Kim, J.S., et al. Mol. Carcinog. 49(10):913-921(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Wilker, E.H., et al. Environ. Health Perspect. 118(7):943-948(2010). Boni, V., et al. Pharmacogenomics J. (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 22748937
• NCBI GeneID: 57510
• NCBI Accession #: NP_065801.1
• NCBI GenBank Nucleotide #: NM_020750.2
• UniProt Accession #: Q9HAV4; Q5JTE6; Q96G48; Q96HN3; Q9BWM6; Q9BZV5; Q9H9M4; Q9NT89; Q9NW39; Q9ULC9
• Molecular Weight: 136,311 Da
• NCBI Official Full Name: exportin-5
• NCBI Official Synonym Full Names: exportin 5
• NCBI Official Symbol: XPO5
• NCBI Official Synonym Symbols: exp5
• NCBI Protein Information: exportin-5
• UniProt Protein Name: Exportin-5
• Protein Family: Exportin
• UniProt Gene Name: XPO5
• UniProt Synonym Gene Names: KIAA1291; RANBP21; Exp5

NCBI Description

This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process. [provided by RefSeq, Aug 2011]

Uniprot Description

Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi). Mediates nuclear export of isoform 5 of ADAR/ADAR1 in a RanGTP-dependent manner.