Urokinase Plasminogen Activator Receptor Recombinant, BSA Free Active Protein
Urokinase Plasminogen Activator Receptor Recombinant, Human, BSA Free (uPAR, CD87)
Applications
ELISA
Purity
>95%, as determined by SDS-PAGE under reducing conditions, visualized by silver stain and quantitative deniometry by blue stain.
Synonyms
Urokinase Plasminogen Activator Receptor Recombinant; BSA Free; Human; BSA Free (uPAR; CD87); BSA Free active protein
Purity/Purification
>95%, as determined by SDS-PAGE under reducing conditions, visualized by silver stain and quantitative deniometry by blue stain.
Form/Format
Supplied as a lyophilized powder from
PBS, pH 7.4. No stabilizing proteins
added. Reconstitute with 1ml sterile PBS.
Concentration
~0.1mg/ml (after reconstitution) (varies by lot)
Applicable Applications for Urokinase Plasminogen Activator Receptor Recombinant, BSA Free active protein
ELISA.
Application Notes
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized rhUPAR at 5ug/ml (100ul/well) can bind rhuPA with a linear range of 0.3-20ng/ml.
Other applications have not been tested. Optimal dilutions to be determined by the researcher.
Measured by its binding ability in a functional ELISA. Immobilized rhUPAR at 5ug/ml (100ul/well) can bind rhuPA with a linear range of 0.3-20ng/ml.
Other applications have not been tested. Optimal dilutions to be determined by the researcher.
Source
NS0-derived, recombinant human uPAR, Lue23-Arg303, with a C-term 6-His tag Swiss/UniProt Accession: Q03405
Endotoxin
<1EU/ug (LAL)
Preparation and Storage
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Urokinase Plasminogen Activator Receptor Recombinant, BSA Free active protein
The urokinase-type plasminogen activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% amino acid sequence identity and the receptor-ligand interaction is strictly species-specific.
Product Categories/Family for Urokinase Plasminogen Activator Receptor Recombinant, BSA Free active protein
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Product Notes
The Urokinase Plasminogen Activator Receptor Recombinant, BSA Free (Catalog #AAA636262) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Urokinase Plasminogen Activator Receptor Recombinant, BSA Free can be used in a range of immunoassay formats including, but not limited to, ELISA. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rhUPAR at 5ug/ml (100ul/well) can bind rhuPA with a linear range of 0.3-20ng/ml. Other applications have not been tested. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Urokinase Plasminogen Activator Receptor Recombinant, BSA Free for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Urokinase Plasminogen Activator Receptor Recombinant, BSA Free, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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