Aurora A Serine/Threonine Kinase, N-terminal His tag Active Protein | aur active protein
Aurora A Serine/Threonine Kinase, Recombinant, Human, N-terminal His tag
Gene Names
aur; 87A7-9/2; aur-A; Aur-A; aurA; AurA; CG3068; ck10; ck[10]; DmelCG3068; l(3)87Ac; l(3)ck10
Reactivity
Human
Purity
90% by SDS-PAGE.
Synonyms
Aurora A Serine/Threonine Kinase; N-terminal His tag; Recombinant; Human; aur active protein
Host
Baculovirus infected Sf9 cells
Reactivity
Human
Purity/Purification
90% by SDS-PAGE.
Form/Format
Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween-20, 50% glycerol, 3mM DTT.
Application Notes
Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Specific Activity
~ 120 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1pmol of Ser/Thr 1 peptide substrate per minute at pH 7.4 and 30 degree C
Assay Buffer
50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na orthovanadate, 0.5mM ATP, 4uM aurora B substrate, and 0.4ug/ml Aurora A kinase.
Preparation and Storage
For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Related Product Information for aur active protein
Human Aurora kinase A (GenBank Accession No. NM_003600), (aa 2-403) with N-terminal HIS6 tag, MW 50kD, expressed in an Baculovirus infected Sf9 cell expression system..
Product Categories/Family for aur active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,228 Da
NCBI Official Full Name
aurora
NCBI Official Synonym Full Names
aurora
NCBI Official Symbol
aur
NCBI Official Synonym Symbols
87A7-9/2; aur-A; Aur-A; aurA; AurA; CG3068; ck10; ck[10]; DmelCG3068; l(3)87Ac; l(3)ck10
NCBI Protein Information
CG3068 gene product from transcript CG3068-RA; A-type aurora kinase; AuroraA; CG3068-PA; aur-PA; aurora A; aurora A kinase; aurora-A; early-A; fruhe2
UniProt Protein Name
Aurora
Protein Family
UniProt Gene Name
aur
UniProt Entry Name
Q9VGF9_DROME
Research Articles on aur
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Product Notes
The aur aur (Catalog #AAA636154) is an Active Protein produced from Baculovirus infected Sf9 cells and is intended for research purposes only. The product is available for immediate purchase. The Aurora A Serine/Threonine Kinase, Recombinant, Human, N-terminal His tag reacts with Human and may cross-react with other species as described in the data sheet. Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Researchers should empirically determine the suitability of the aur aur for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aurora A Serine/Threonine Kinase, N-terminal His tag, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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