Rabbit anti-Human ULBP2 Polyclonal Antibody | anti-ULBP2 antibody

ULBP2, ID (ULBP2, N2DL2, RAET1H, NKG2D ligand 2, ALCAN-alpha, Retinoic acid early transcript 1H, UL16-binding protein 2) (PE)

Cat. Num. AAA6361288

• Gene Names: ULBP2; N2DL2; RAET1H; NKG2DL2; ALCAN-alpha
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ULBP2; Polyclonal Antibody; ID (ULBP2; N2DL2; RAET1H; NKG2D ligand 2; ALCAN-alpha; Retinoic acid early transcript 1H; UL16-binding protein 2) (PE); UL16-binding protein 2; anti-ULBP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ULBP2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ULBP2 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 88-116 in the central region of human ULBP2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in U251 cell line lysates (35ug/lane) usingMBS6009406. This demonstrates that MBS6009406 detected the ULBP2 protein (arrow).)

Western Blot (WB)

(Western Blot analysis of ULBP2 (arrow) in 293 cell lysates (2 ug/lane) using MBS6009406 either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ULBP2 gene.)

Related Product Information for anti-ULBP2 antibody

Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface.

Product Categories/Family for anti-ULBP2 antibody

Antibodies; Binding Proteins

References

1. McGilvray, R.W., et al. Int. J. Cancer 127(6):1412-1420(2010). 2. Nuckel, H., et al. Leukemia 24(6):1152-1159(2010). 3. Antoun, A., et al. Hum. Immunol. 71(6):610-620(2010). 4. Davila, S., et al. Genes Immun. 11(3):232-238(2010). 5. Ward, J., et al. PLoS Pathog. 5 (10), E1000613 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 13376824
• NCBI GeneID: 80328
• NCBI Accession #: NP_079493.1
• NCBI GenBank Nucleotide #: NM_025217.3
• UniProt Accession #: Q9BZM5; Q5VUN4
• Molecular Weight: 27,368 Da
• NCBI Official Full Name: NKG2D ligand 2 preproprotein
• NCBI Official Synonym Full Names: UL16 binding protein 2
• NCBI Official Symbol: ULBP2
• NCBI Official Synonym Symbols: N2DL2; RAET1H; NKG2DL2; ALCAN-alpha
• NCBI Protein Information: NKG2D ligand 2; retinoic acid early transcript 1H
• UniProt Protein Name: NKG2D ligand 2
• Protein Family: NKG2D ligand
• UniProt Gene Name: ULBP2
• UniProt Synonym Gene Names: N2DL2; RAET1H; N2DL-2; NKG2DL2
• UniProt Entry Name: N2DL2_HUMAN

NCBI Description

This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in turn contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells. This gene is located in a cluster of multiple MHC class I-related genes on chromosome 6. [provided by RefSeq, Jul 2015]