Rabbit anti-Human TUBG1 Polyclonal Antibody | anti-TUBG1 antibody

TUBG1, NT (TUBG1, TUBG, Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1) (PE)

Cat. Num. AAA6360221

• Gene Names: TUBG1; TUBG; GCP-1; TUBGCP1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TUBG1; Polyclonal Antibody; NT (TUBG1; TUBG; Tubulin gamma-1 chain; Gamma-1-tubulin; Gamma-tubulin complex component 1) (PE); Gamma-tubulin complex component 1; anti-TUBG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TUBG1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TUBG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human TUBG1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TUBG1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the TUBG1 antibody detected the TUBG1 protein (arrow).)

Related Product Information for anti-TUBG1 antibody

This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7.

Product Categories/Family for anti-TUBG1 antibody

Antibodies; Tubulin

References

Olson, J.E., et al. Breast Cancer Res. Treat. (2010). In press: Couch, F.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(1):251-257(2010). Alvarado-Kristensson, M., et al. Nat. Cell Biol. 11(9):1081-1092(2009). Zhang, X., et al. J. Cell. Sci. 122 (PT 13), 2240-2251 (2009): Haren, L., et al. PLoS ONE 4 (6), E5976 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 31543831
• NCBI GeneID: 7283
• NCBI Accession #: NP_001061.2
• NCBI GenBank Nucleotide #: NM_001070.4
• UniProt Accession #: P23258; Q53X79; Q9BW59
• Molecular Weight: 51,170 Da
• NCBI Official Full Name: tubulin gamma-1 chain
• NCBI Official Synonym Full Names: tubulin, gamma 1
• NCBI Official Symbol: TUBG1
• NCBI Official Synonym Symbols: TUBG; GCP-1; TUBGCP1
• NCBI Protein Information: tubulin gamma-1 chain; gamma-1-tubulin; tubulin, gamma polypeptide; gamma-tubulin complex component 1
• UniProt Protein Name: Tubulin gamma-1 chain
• UniProt Gene Name: TUBG1
• UniProt Synonym Gene Names: TUBG; GCP-1
• UniProt Entry Name: TBG1_HUMAN

NCBI Description

This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]

Uniprot Description

TUBG1: Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Interacts with GCP2 and GCP3. Interacts with B9D2. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO/C1orf88. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: centriole; centrosome; recycling endosome; pericentriolar material; condensed nuclear chromosome; cytoplasmic microtubule; nonmotile primary cilium; apical part of cell; cytoplasm; leading edge; polar microtubule; cytosol; gamma-tubulin complex

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: metabolic process; organelle organization and biogenesis; mitotic cell cycle; microtubule cytoskeleton organization and biogenesis; microtubule nucleation; G2/M transition of mitotic cell cycle; cytoplasmic microtubule organization and biogenesis; meiotic spindle organization and biogenesis

Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 4