Interleukin 10 Active Protein | IL10 active protein

Interleukin 10, Recombinant, Human (Interleukin-10, IL-10, IL10, IL10X, Cytokine Synthesis Inhibitory Factor, CSIF)

Cat. Num. AAA635982

• Gene Names: IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
• Purity: Highly Purified; ~95% (SDS-PAGE)
• Synonyms: Interleukin 10; Recombinant; Human (Interleukin-10; IL-10; IL10; IL10X; Cytokine Synthesis Inhibitory Factor; CSIF); IL10 active protein

• Purity/Purification: Highly Purified; ~95% (SDS-PAGE)
• Form/Format: Supplied as a lyophilized powder in PBS. Reconstitute with 100ug/ml in PBS.

• Biological Activity: Measured by its ability to inhibit IL10dependent proliferation of MC/92 mouse mast cells. ThompsonSnipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is typically 0.05 0.25ug/ml in the presence of 2ng/ml of recombinant human IL10.
• Endotoxin Level: <1EU/ug (LAL method)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffe. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for IL10 active protein

Interleukin10 Receptor alpha (IL10 Ra), also known as IL10 R1, is a 90 110kD transmembrane glycoprotein member of the class II cytokine receptor family (1). IL10

Product Categories/Family for IL10 active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 10835141
• NCBI GeneID: 3586
• NCBI Accession #: NP_000563.1
• NCBI GenBank Nucleotide #: NM_000572.2
• UniProt Accession #: P22301
• Molecular Weight: ~24.4kD
• NCBI Official Full Name: interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: IL10
• NCBI Official Synonym Symbols: CSIF; TGIF; GVHDS; IL-10; IL10A
• NCBI Protein Information: interleukin-10; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor
• UniProt Protein Name: Interleukin-10
• Protein Family: Interleukin
• UniProt Gene Name: IL10
• UniProt Synonym Gene Names: IL-10; CSIF
• UniProt Entry Name: IL10_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]

Uniprot Description

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.

Protein type: Motility/polarity/chemotaxis; Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; interleukin-10 receptor binding

Biological Process: B cell proliferation; negative regulation of chronic inflammatory response to antigenic stimulus; regulation of isotype switching; positive regulation of transcription, DNA-dependent; leukocyte chemotaxis; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; response to insulin stimulus; negative regulation of interferon-alpha biosynthetic process; cytoplasmic sequestering of NF-kappaB; negative regulation of T cell proliferation; cell-cell signaling; negative regulation of interferon-gamma production; positive regulation of MHC class II biosynthetic process; response to molecule of bacterial origin; negative regulation of interleukin-6 production; negative regulation of tumor necrosis factor biosynthetic process; hemopoiesis; inflammatory response; aging; response to drug; response to inactivity; negative regulation of interleukin-12 production; T-helper 2 type immune response; positive regulation of B cell apoptosis; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of cytokine secretion during immune response; regulation of gene expression; B cell differentiation; defense response to bacterium; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; regulation of sensory perception of pain; receptor biosynthetic process; response to activity; negative regulation of apoptosis; positive regulation of cytokine secretion

Disease: Graft-versus-host Disease, Susceptibility To; Rheumatoid Arthritis; Human Immunodeficiency Virus Type 1, Susceptibility To