Rabbit anti-Human TSPYL6 Polyclonal Antibody | anti-TSPYL6 antibody

TSPYL6, ID (TSPYL6, Testis-specific Y-encoded-like protein 6) (Biotin)

Cat. Num. AAA6359729

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TSPYL6; Polyclonal Antibody; ID (TSPYL6; Testis-specific Y-encoded-like protein 6) (Biotin); Testis-specific Y-encoded-like protein 6; anti-TSPYL6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TSPYL6 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IF: 1:10-50; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TSPYL6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213-243 amino acids from the Central region of human TSPYL6.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(TSPYL6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSPYL6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TSPYL6 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of TSPYL6 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

Western Blot (WB)

(TSPYL6 Antibody (Center) western blot analysis in NCI-H460,Hela cell line lysates (35ug/lane).This demonstrates the TSPYL6 antibody detected the TSPYL6 protein (arrow).)

Product Categories/Family for anti-TSPYL6 antibody

Antibodies; Spermatogenesis; Testis Proteins

References

Kimura, K., et al. Genome Res. 16(1):55-65(2006). Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 146198777
• NCBI GeneID: 388951
• NCBI Accession #: NP_001003937.2
• NCBI GenBank Nucleotide #: NM_001003937.2
• UniProt Accession #: Q8N831; Q6NUJ3
• Molecular Weight: 45,874 Da
• NCBI Official Full Name: testis-specific Y-encoded-like protein 6
• NCBI Official Synonym Full Names: TSPY-like 6
• NCBI Official Symbol: TSPYL6
• NCBI Protein Information: testis-specific Y-encoded-like protein 6; TSPY-like protein 6
• UniProt Protein Name: Testis-specific Y-encoded-like protein 6
• Protein Family: Testis-specific Y-encoded-like protein
• UniProt Gene Name: TSPYL6
• UniProt Synonym Gene Names: TSPY-like protein 6
• UniProt Entry Name: TSYL6_HUMAN