Integrin alphaM, beta2 Active Protein
Integrin alphaM, beta2, Recombinant, Human (Mac-1, CD11b/CD18)
Purity
>95%, as determined by SDS-PAGE and visualized by silver stain.
Synonyms
Integrin alphaM; beta2; Recombinant; Human (Mac-1; CD11b/CD18); beta2 active protein
Host
Chinese hamster ovary (CHO) cells
Specificity
The 1088aa human aM/CD11b ECD shares 73-76% aa sequence identity with mouse, rat, bovine and canine aM while the 678aa human b2/CD18 ECD shares 81-83% aa sequence identity with mouse, rat, bovine, canine, goat, sheep, and porcine b2.
Purity/Purification
>95%, as determined by SDS-PAGE and visualized by silver stain.
Form/Format
Supplied as lyophillized powder from 50mM Tris, 0.15M sodium chloride, 1mM MgCl2, pH7.4. Reconstitute with sterile PBS to 0.1mg/ml.
Activity
Measured by the ability of the immobilized protein to support the adhesion of CHO cells transfected with human ICAM-1. The ED50 for this effect is 0.25-1.5ug/ml.
Endotoxin
< 1EU/ug (LAL).
Molecular Mass
Based on N-terminal sequencing, the recombinant human Integrin aM subunit starts at Phe 17 and has a calculated molecular mass of ~124.7kD. The N-terminal sequence of the b2 subunit was blocked, suggesting that the b2 subunit may start at the predicted Gln 23 and has a calculated molecular mass of a~79kD. As a result of glycosylation, the recombinant proteins migrate as ~165 and 100kD protein bands in SDS-PAGE under reducing conditions.
Preparation and Storage
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Integrin alphaM, beta2 active protein
DNA sequences encoding the extracellular domain of human Integrin alpha M (Phe17-Asn1105; Accession# P11215) (Corbi, A.L., et al., 1988, J. Biol. Chem. 263:12403) with an acidic tail at the C-terminus and the extracellular domain of human Integrin b2 (Gln23-Asn700; Accession# P05107) (Kishimoto, T. et al., 1987, Cell 48:681) with a basic tail at the C-terminus were cloned downstream of the human CD33 signal peptides and co-expressed in Chinese hamster ovary (CHO) cells.
Product Categories/Family for Integrin alphaM, beta2 active protein
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Product Notes
The Integrin alphaM, beta2 (Catalog #AAA635841) is an Active Protein produced from Chinese hamster ovary (CHO) cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Integrin alphaM, beta2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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