Rabbit anti-Human TRIM2 Polyclonal Antibody | anti-TRIM2 antibody

TRIM2, CT (TRIM2, KIAA0517, RNF86, Tripartite motif-containing protein 2, E3 ubiquitin-protein ligase TRIM2, RING finger protein 86) (PE)

Cat. Num. AAA6358186

• Gene Names: TRIM2; CMT2R; RNF86
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRIM2; Polyclonal Antibody; CT (TRIM2; KIAA0517; RNF86; Tripartite motif-containing protein 2; E3 ubiquitin-protein ligase TRIM2; RING finger protein 86) (PE); RING finger protein 86; anti-TRIM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TRIM2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TRIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 591-621 amino acids from the C-terminal region of human TRIM2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TRIM2 Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the TRIM2 antibody detected the TRIM2 protein (arrow).)

Related Product Information for anti-TRIM2 antibody

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified. [provided by RefSeq].

Product Categories/Family for anti-TRIM2 antibody

Antibodies; Transcription Factors; TRIM (Tripartite Motif)

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Ohkawa, N., et al. J. Neurochem. 78(1):75-87(2001). Reymond, A., et al. EMBO J. 20(9):2140-2151(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 194248079
• NCBI GeneID: 23321
• NCBI Accession #: NP_056086.2; NP_001123539.1
• NCBI GenBank Nucleotide #: NM_015271.4
• UniProt Accession #: Q9C040; O60272; Q9BSI9; Q9UFZ1; D3DP09
• Molecular Weight: 84,545 Da
• NCBI Official Full Name: tripartite motif-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: tripartite motif containing 2
• NCBI Official Symbol: TRIM2
• NCBI Official Synonym Symbols: CMT2R; RNF86
• NCBI Protein Information: tripartite motif-containing protein 2; RING finger protein 86; tripartite motif protein TRIM2; E3 ubiquitin-protein ligase TRIM2
• UniProt Protein Name: Tripartite motif-containing protein 2
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM2
• UniProt Synonym Gene Names: KIAA0517; RNF86
• UniProt Entry Name: TRIM2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. It plays a neuroprotective role and functions as an E3-ubiquitin ligase in proteasome-mediated degradation of target proteins. Mutations in this gene can cause early-onset axonal neuropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Uniprot Description

TRIM2: UBE2D1-dependent E3 ubiquitin-protein ligase that mediates the ubiquitination of NEFL and of phosphorylated BCL2L11. Plays a neuroprotective function. May play a role in neuronal rapid ischemic tolerance. Belongs to the TRIM/RBCC family.

Protein type: Ubiquitin conjugating system; EC 6.3.2.-; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: cytoplasm

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: regulation of neuron apoptosis; protein ubiquitination

Disease: Charcot-marie-tooth Disease, Axonal, Type 2r