Heparinase II, P. heparinus Active Protein
Heparinase II, Recombinant, P. heparinus
Purity
Highly Purified~95% (SDS-PAGE, reducing conditions; 5ug protein loaded per lane; visualized by colloidal Coomassie blue staining)
Synonyms
Heparinase II; P. heparinus; Recombinant; P. heparinus active protein
Purity/Purification
Highly Purified
~95% (SDS-PAGE, reducing conditions; 5ug protein loaded per lane; visualized by colloidal Coomassie blue staining)
~95% (SDS-PAGE, reducing conditions; 5ug protein loaded per lane; visualized by colloidal Coomassie blue staining)
Form/Format
Supplied as a sterile-filtered liquid in Tris-buffered saline
Specific Activity
Measured by its ability to liberate oligosaccharides from heparin. The specific activity is >750 pmoles/min/ug, as measured under the described conditions (see Activity Assay Protocol)
Endotoxin Level
<1EU/ug (LAL method)
Preparation and Storage
Aliquot to avoid repeated freezing and thawing. Store at -70 degree C. Aliquots are stable for 6 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Heparinase II, P. heparinus active protein
Heparan sulfate is a sulfated glycosaminoglycan with the repeating disaccharide units of 4HexA1, 4GlcNAcB1. It is usually attached to the protein cores of proteoglycans found on cell membrane and extracellular matrix, where it binds to a variety of protein ligands and regulates a wide range of biological activities, including developmental processes, angiogenesis, blood coagulation and tumor metastasis (1, 2). Heparan sulfate has a domain structure containing sulfated regions interspaced with less or nonsulfated regions (3, 4). Heparin shares the backbone structure with heparan sulfate but contains no nonsulfated regions. Heparinases are a family of lyases that release unsaturated oligosaccharides from heparin and heparan sulfate upon digestion (5). Heparinase I recognizes highly sulfated regions and is more specific for heparin. Heparinase II digests both heparin and heparan sulfate. Heparinase III prefers less sulfated regions and is active only on heparan sulfate (6, 7).
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Product Notes
The Heparinase II, P. heparinus (Catalog #AAA635728) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Heparinase II, P. heparinus, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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