Ku, p70, p80 Recombinant Protein | XRCC5 recombinant protein

Ku, p70, p80, Recombinant, Human

Cat. Num. AAA635725

• Gene Names: XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
• Purity: Highly Purified; 90% (SDS-PAGE)
• Synonyms: Ku; p70; p80; Recombinant; Human; XRCC5 recombinant protein

• Host: Sf9 insect cells
• Purity/Purification: Highly Purified; 90% (SDS-PAGE)
• Form/Format: Supplied as a liquid in 16mM HEPES buffer, pH 8.0, 250mM sodium chloride and 20% glycerol.

• Application Notes: Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera.
• Preparation and Storage: -20 degree C; Stability: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Please avoid freeze-thaw cycles.

Related Product Information for XRCC5 recombinant protein

Ku antigen consists of two polypeptides (70kD and 80kD; the p80 subunit is occasionally called p86). The Ku heterodimer provides DNA-binding and regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V(D)J recombination, telomere maintenance, and the regulation of specific gene transcription. Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome (up to 20% of patients) but have also been detectable in about 20 % of patients with primary pulmonary hypertension (as a complication of overlap), in 5-10 % of SLE and MCTD patients, and in up to 20 % of patients with primary Sjögren's syndrome. In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent.

Recombinant Human KuP70/P80 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 43,591 dalton. Recombinant Ku(P70/P80) is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Product Categories/Family for XRCC5 recombinant protein

Molecular Biology; MB-Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 307094
• NCBI GeneID: 7520
• UniProt Accession #: P13010; Q0Z7V0; Q4VBQ5; Q53HH7; Q7M4N0; Q9UCQ0; Q9UCQ1; A8K3X5
• Molecular Weight: 43.59kD
• NCBI Official Full Name: Ku (p70/p80) subunit
• NCBI Official Synonym Full Names: X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
• NCBI Official Symbol: XRCC5
• NCBI Official Synonym Symbols: KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
• NCBI Protein Information: X-ray repair cross-complementing protein 5; TLAA; CTC85; CTCBF; nuclear factor IV; Ku autoantigen, 80kDa; DNA repair protein XRCC5; thyroid-lupus autoantigen; 86 kDa subunit of Ku antigen; lupus Ku autoantigen protein p86; Ku86 autoantigen related protein 1; CTC box-binding factor 85 kDa subunit; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit
• UniProt Protein Name: X-ray repair cross-complementing protein 5
• UniProt Gene Name: XRCC5
• UniProt Synonym Gene Names: G22P2; CTC85; CTCBF; TLAA
• UniProt Entry Name: XRCC5_HUMAN

NCBI Description

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]

Uniprot Description

Ku80: the 80-kilodalton subunit of the Ku complex, also known as ATP-dependant DNA helicase II. A single stranded DNA-dependent ATP-dependent helicase. It functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70.

Protein type: Helicase; EC 3.6.1.-; DNA-binding; Nucleolus; Nuclear receptor co-regulator; RNA-binding; EC 3.6.4.-

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleoplasm; nuclear telomere cap complex; nuclear chromosome, telomeric region; membrane; plasma membrane; nucleolus; cytosol; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; telomeric DNA binding; damaged DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity; ATP binding

Biological Process: response to drug; positive regulation of neurogenesis; viral reproduction; transcription, DNA-dependent; DNA repair; DNA duplex unwinding; double-strand break repair via nonhomologous end joining; DNA recombination; cell proliferation; double-strand break repair; positive regulation of interferon type I production; innate immune response; brain development; negative regulation of transcription, DNA-dependent; telomere maintenance