Netrin-1 Active Protein | NTN1 active protein

Netrin-1, Recombinant, Human (NTN1)

Cat. Num. AAA635618

• Gene Names: NTN1; NTN1L
• Purity: >=95%
• Synonyms: Netrin-1; Recombinant; Human (NTN1); NTN1 active protein

• Specificity: Binds to human, mouse and rat UNC5B.
• Purity/Purification: >=95%
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with protein stabilizer. No BSA included. The protein stabilizer shows no interference with protein activity. Reconstitute with 100ul sterile ddH2O
• Concentration: ~0.1 mg/ml (after reconstitution) (varies by lot)
• Sequence: Human netrin-1 (aa 25-604) is fused at the N-terminus to a DYKDDDDK-tag.

• Grade: Highly Purified
• Source: Human, HEK 293 cells
• Biological Activity: Induces axon outgrowth
• Endotoxin: < 0.01 EU/ug protein (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Futher dilutions can be made in assay buffer.

Related Product Information for NTN1 active protein

Netrin-1 controls guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Netrin-1 is also Involved in tumorigenesis by regulating apoptosis. Recombinant protein corresponding to aa25-604 with FLAG®-tag at the C-Terminus of human NTN1.

Product Categories/Family for NTN1 active protein

Molecular Biology; MB-Netrin

NCBI and Uniprot Product Information

• NCBI GI #: 148613884
• NCBI GeneID: 9423
• NCBI Accession #: NP_004813.2
• NCBI GenBank Nucleotide #: NM_004822.2
• UniProt Accession #: O95631; E9KL51
• Molecular Weight: ~80kD (SDS-PAGE)
• NCBI Official Full Name: netrin-1
• NCBI Official Synonym Full Names: netrin 1
• NCBI Official Symbol: NTN1
• NCBI Official Synonym Symbols: NTN1L
• NCBI Protein Information: netrin-1; netrin 1, mouse, homolog of; epididymis tissue protein Li 131P
• UniProt Protein Name: Netrin-1
• Protein Family: Netrin
• UniProt Gene Name: NTN1
• UniProt Synonym Gene Names: NTN1L
• UniProt Entry Name: NET1_HUMAN

NCBI Description

Netrin is included in a family of laminin-related secreted proteins. The function of this gene has not yet been defined; however, netrin is thought to be involved in axon guidance and cell migration during development. Mutations and loss of expression of netrin suggest that variation in netrin may be involved in cancer development. [provided by RefSeq, Jul 2008]

Uniprot Description

NTN1: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasm; extracellular region; basement membrane

Molecular Function: protein binding

Biological Process: axon guidance; inner ear morphogenesis; cell-cell adhesion; establishment of nucleus localization; apoptosis; positive regulation of axon extension; positive regulation of cell proliferation; neuron migration; negative regulation of axon extension; regulation of cell migration; anterior/posterior axon guidance