Rabbit anti-Mouse Tlk1 Polyclonal Antibody | anti-Tlk1 antibody

Tlk1, CT (Tlk1, Serine/threonine-protein kinase tousled-like 1, Tousled-like kinase 1) (FITC)

Cat. Num. AAA6355825

• Gene Names: Tlk1; 4930545J15Rik
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Tlk1; Polyclonal Antibody; CT (Tlk1; Serine/threonine-protein kinase tousled-like 1; Tousled-like kinase 1) (FITC); Tousled-like kinase 1; anti-Tlk1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Tlk1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Tlk1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 700-728 amino acids from the C-terminal region of Mouse Tlk1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Tlk1 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Tlk1 antibody detected the Tlk1 protein (arrow).)

Related Product Information for anti-Tlk1 antibody

Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly (By similarity). Isoform 3 protects the cells from the ionizing radiation by faciliting the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10' (By similarity).

Product Categories/Family for anti-Tlk1 antibody

Antibodies; Protein Kinases

References

Canfield, C., et al. BMC Mol. Biol. 10, 110 (2009): Taniwaki, T., et al. Dev. Growth Differ. 47(3):163-172(2005). Sunavala-Dossabhoy, G., et al. BMC Mol. Biol. 6, 19 (2005): Cobellis, G., et al. Nucleic Acids Res. 33 (4), E44 (2005): Okazaki, N., et al. DNA Res. 11(3):205-218(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 224994273
• NCBI GeneID: 228012
• NCBI Accession #: NP_766252.2
• NCBI GenBank Nucleotide #: NM_172664.3
• UniProt Accession #: Q8C0V0
• Molecular Weight: 86,624 Da
• NCBI Official Full Name: serine/threonine-protein kinase tousled-like 1
• NCBI Official Synonym Full Names: tousled-like kinase 1
• NCBI Official Symbol: Tlk1
• NCBI Official Synonym Symbols: 4930545J15Rik
• NCBI Protein Information: serine/threonine-protein kinase tousled-like 1
• UniProt Protein Name: Serine/threonine-protein kinase tousled-like 1
• Protein Family: Serine/threonine-protein kinase tousled
• UniProt Gene Name: Tlk1
• UniProt Entry Name: TLK1_MOUSE

Uniprot Description

Function: Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly

By similarity. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'

By similarity. Ref.4

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium

By similarity.

Enzyme regulation: Cell-cycle regulated, maximal activity in S-phase. Inactivated by phosphorylation at Ser-743, potentially by CHEK1

By similarity.

Subunit structure: Heterodimerizes with TLK2. Interacts with ASF1A and ASF1B

By similarity.

Subcellular location: Nucleus

By similarity.

Tissue specificity: Ubiquitously expressed in all tissues examined. Ref.3

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Contains 1 protein kinase domain.

Sequence caution: The sequence AAH51641.1 differs from that shown. Reason: Erroneous initiation.