Glutathione S Transferase, T1-1 Human Active Protein | GSTT1 active protein

Glutathione S Transferase, T1-1, Recombinant Human (GST)

Cat. Num. AAA635557

• Purity: ~95% (SDS-PAGE); Specifications: >95%
• Synonyms: Glutathione S Transferase; T1-1 Human; T1-1; Recombinant Human (GST); GSTT1 active protein

• Specificity: This purified product exhibits high enzymatic activity for CDNB, the synthetic substrate that is most commonly used for GST analyses. However, as is widely known for GST activity analysis using. CDNB as a substrate, low concentrations (high dilutions) of the enzyme result in lower activity values.
• Purity/Purification: ~95% (SDS-PAGE); Specifications: >95%
• Form/Format: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 50mM sodium chloride, 1mM DTT, 5mM EDTA and 50% glycerol.
• Concentration: ~1mg/ml; Specifications: 2.5mg/ml (varies by lot)

• Biological Activity: ~30 Units/mg using spectrophotometric determination of 4- nitrobenzyl chloride conjugation with reduced glutathione (5mM) in 100mM NaPO4 (pH 6.5) at room temperature.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Categories/Family for GSTT1 active protein

BioSeparation; BioSep-Assay Detection; Tags

NCBI and Uniprot Product Information

• NCBI GI #: 167466164
• NCBI GeneID: 2952
• NCBI Accession #: NP_000844.2
• NCBI GenBank Nucleotide #: NM_000853.2
• UniProt Accession #: P30711; O00226; Q5TZY2; Q969K8; Q96IY3
• Molecular Weight: ~25kD
• NCBI Official Full Name: glutathione S-transferase theta-1
• NCBI Official Synonym Full Names: glutathione S-transferase theta 1
• NCBI Official Symbol: GSTT1
• NCBI Protein Information: glutathione S-transferase theta-1; GST class-theta-1; glutathione transferase T1-1
• UniProt Protein Name: Glutathione S-transferase theta-1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTT1
• UniProt Entry Name: GSTT1_HUMAN

NCBI Description

Glutathione S-transferase (GST) theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTT1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide. Belongs to the GST superfamily. Theta family.

Protein type: Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 2.5.1.18; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: cytosol

Molecular Function: glutathione transferase activity; glutathione peroxidase activity

Biological Process: glutathione metabolic process; xenobiotic metabolic process