Rabbit anti-Human SRA1 Polyclonal Antibody | anti-SRA1 antibody

SRA1, CT (SRA1, Steroid receptor RNA activator 1, Steroid receptor RNA activator protein) (PE)

Cat. Num. AAA6351025

• Gene Names: SRA1; SRA; SRAP; STRAA1; pp7684
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SRA1; Polyclonal Antibody; CT (SRA1; Steroid receptor RNA activator 1; Steroid receptor RNA activator protein) (PE); Steroid receptor RNA activator protein; anti-SRA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SRA1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SRA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-222 amino acids from the C-terminal region of human SRA1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SRA1 Antibody (C-term) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the SRA1 antibody detected the SRA1 protein (arrow).)

Related Product Information for anti-SRA1 antibody

This gene is involved in transcriptional coactivation by steroid receptors. There is currently data suggesting this gene encodes both a non-coding RNA that functions as part of a ribonucleoprotein complex and a protein coding mRNA. Increased expression of both the transcript and the protein is associated with cancer.

Product Categories/Family for anti-SRA1 antibody

Antibodies; Receptors

References

Yao, H., et al. Genes Dev. 24(22):2543-2555(2010). Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Beauvillain, C., et al. J. Hepatol. 52(5):644-651(2010). Foulds, C.E., et al. Mol. Endocrinol. 24(5):1090-1105(2010). Chooniedass-Kothari, S., et al. FEBS Lett. 584(6):1174-1180(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 113951734
• NCBI GeneID: 10011
• NCBI Accession #: NP_001030312.2
• NCBI GenBank Nucleotide #: NM_001035235.3
• UniProt Accession #: Q9HD15; Q6NVU9; Q8IXM1; Q9HD13; Q9HD14
• Molecular Weight: 25,673 Da
• NCBI Official Full Name: steroid receptor RNA activator 1 isoform 1
• NCBI Official Synonym Full Names: steroid receptor RNA activator 1
• NCBI Official Symbol: SRA1
• NCBI Official Synonym Symbols: SRA; SRAP; STRAA1; pp7684
• NCBI Protein Information: steroid receptor RNA activator 1; SRA1; steroid receptor RNA activator 1 (complexes with NCOA1); steroid receptor RNA activator protein; steroid receptor coactivator
• UniProt Protein Name: Steroid receptor RNA activator 1
• Protein Family: Steroid receptor RNA activator
• UniProt Gene Name: SRA1
• UniProt Synonym Gene Names: SRAP
• UniProt Entry Name: SRA1_HUMAN

NCBI Description

Both long non-coding and protein-coding RNAs are transcribed from this gene, and they represent alternatively spliced transcript variants. This gene was initially defined as a non-coding RNA, which is a coactivator for several nuclear receptors (NRs) and is associated with breast cancer. It has now been found that this gene is involved in the regulation of many NR and non-NR activities, including metabolism, adipogenesis and chromatin organization. The long non-coding RNA transcripts interact with a variety of proteins, including the protein encoded by this gene. The encoded protein acts as a transcriptional repressor by binding to the non-coding RNA. [provided by RefSeq, Mar 2012]

Uniprot Description

SRA1: Functional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand- dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis. Belongs to the SRA1 family.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: nucleoplasm; microtubule cytoskeleton; intercellular bridge; cytoplasm; plasma membrane; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; ligand-dependent nuclear receptor transcription coactivator activity; transcription coactivator activity

Biological Process: regulation of apoptosis; cell proliferation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis; cell differentiation