Western Blot (WB)
(SPOPL Antibody (Center) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the SPOPL antibody detected the SPOPL protein (arrow).)
Rabbit anti-Human SPOPL Polyclonal Antibody | anti-SPOPL antibody
SPOPL, ID (SPOPL, Speckle-type POZ protein-like, HIB homolog 2, Roadkill homolog 2) (PE)
Gene Names
SPOPL; BTBD33
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SPOPL; Polyclonal Antibody; ID (SPOPL; Speckle-type POZ protein-like; HIB homolog 2; Roadkill homolog 2) (PE); Roadkill homolog 2; anti-SPOPL antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-SPOPL antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SPOPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-357 amino acids from the Central region of human SPOPL.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(SPOPL Antibody (Center) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the SPOPL antibody detected the SPOPL protein (arrow).)
Western Blot (WB)
(SPOPL Antibody (Center) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the SPOPL antibody detected the SPOPL protein (arrow).)
Immunohistochemistry (IHC)
(SPOPL Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPOPL Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SPOPL Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPOPL Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(SPOPL Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SPOPL Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-SPOPL antibody
In complex with a cullin, may act in ubiquitination and proteasomal degradation processes (By similarity).
Product Categories/Family for anti-SPOPL antibody
References
Lamesch, P., et al. Genomics 89(3):307-315(2007). Zhang, Q., et al. Dev. Cell 10(6):719-729(2006). Kent, D., et al. Development 133(10):2001-2010(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,647 Da
NCBI Official Full Name
speckle-type POZ protein-like
NCBI Official Synonym Full Names
speckle-type POZ protein-like
NCBI Official Symbol
SPOPL
NCBI Official Synonym Symbols
BTBD33
NCBI Protein Information
speckle-type POZ protein-like; HIB homolog 2; roadkill homolog 2
UniProt Protein Name
Speckle-type POZ protein-like
Protein Family
UniProt Gene Name
SPOPL
UniProt Entry Name
SPOPL_HUMAN
Uniprot Description
SPOPL: In complex with a cullin, may act in ubiquitination and proteasomal degradation processes. Belongs to the Tdpoz family.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 2q22.1
Cellular Component: nucleus
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; negative regulation of protein ubiquitination
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Product Notes
The SPOPL spopl (Catalog #AAA6350750) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPOPL, ID (SPOPL, Speckle-type POZ protein-like, HIB homolog 2, Roadkill homolog 2) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPOPL can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SPOPL spopl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPOPL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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