Rabbit anti-Human SNAP23 Polyclonal Antibody | anti-SNAP23 antibody

SNAP23, CT (SNAP23, Synaptosomal-associated protein 23, Vesicle-membrane fusion protein SNAP-23) (Azide free) (HRP)

Cat. Num. AAA6349214

• Gene Names: SNAP23; SNAP-23; SNAP23A; SNAP23B; HsT17016
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SNAP23; Polyclonal Antibody; CT (SNAP23; Synaptosomal-associated protein 23; Vesicle-membrane fusion protein SNAP-23) (Azide free) (HRP); Vesicle-membrane fusion protein SNAP-23; anti-SNAP23 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SNAP23 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SNAP23 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-199 amino acids from the C-terminal region of human SNAP23.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SNAP23 Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the SNAP23 antibody detected the SNAP23 protein (arrow).)

Western Blot (WB)

(Western blot analysis of SNAP23 (arrow) using rabbit polyclonal SNAP23 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SNAP23 gene.)

Immunohistochemistry (IHC)

(SNAP23 Antibody (C-term) (AP14590b)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SNAP23 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SNAP23 antibody

Specificity of vesicular transport is regulated, in part, by the interaction of a vesicle-associated membrane protein termed synaptobrevin/VAMP with a target compartment membrane protein termed syntaxin. These proteins, together with SNAP25 (synaptosome-associated protein of 25 kDa), form a complex which serves as a binding site for the general membrane fusion machinery. Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is present almost exclusively in the brain, suggesting that a ubiquitously expressed homolog of SNAP25 exists to facilitate transport vesicle/target membrane fusion in other tissues. The protein encoded by this gene is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternative transcript variants encoding different protein isoforms have been described for this gene.

Product Categories/Family for anti-SNAP23 antibody

Antibodies; Membrane Proteins

References

Greaves, J., et al. J. Biol. Chem. 285(32):24629-24638(2010). Bostrom, P., et al. Diabetes 59(8):1870-1878(2010). Ban, H.J., et al. BMC Genet. 11, 26 (2010): Kean, M.J., et al. J. Cell. Sci. 122 (PT 22), 4089-4098 (2009): Gratacos, M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (6), 808-816 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 18765729
• NCBI GeneID: 8773
• NCBI Accession #: NP_003816.2; NP_570710.1
• NCBI GenBank Nucleotide #: NM_003825.3
• UniProt Accession #: O00161; O00162; Q13602; Q6IAE3
• Molecular Weight: 17,789 Da
• NCBI Official Full Name: synaptosomal-associated protein 23 isoform SNAP23A
• NCBI Official Synonym Full Names: synaptosomal-associated protein, 23kDa
• NCBI Official Symbol: SNAP23
• NCBI Official Synonym Symbols: SNAP-23; SNAP23A; SNAP23B; HsT17016
• NCBI Protein Information: synaptosomal-associated protein 23; synaptosomal-associated protein, 23kD; vesicle-membrane fusion protein SNAP-23
• UniProt Protein Name: Synaptosomal-associated protein 23
• Protein Family: Synaptosomal-associated protein
• UniProt Gene Name: SNAP23
• UniProt Synonym Gene Names: SNAP-23
• UniProt Entry Name: SNP23_HUMAN

NCBI Description

Specificity of vesicular transport is regulated, in part, by the interaction of a vesicle-associated membrane protein termed synaptobrevin/VAMP with a target compartment membrane protein termed syntaxin. These proteins, together with SNAP25 (synaptosome-associated protein of 25 kDa), form a complex which serves as a binding site for the general membrane fusion machinery. Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is present almost exclusively in the brain, suggesting that a ubiquitously expressed homolog of SNAP25 exists to facilitate transport vesicle/target membrane fusion in other tissues. The protein encoded by this gene is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SNAP23: a member of the SNAP-25 family of SNARE proteins. Structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternatively spliced isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: nucleoplasm; SNARE complex; azurophil granule; specific granule; neuron projection; focal adhesion; mast cell granule; cytoplasm; plasma membrane; synapse

Molecular Function: SNAP receptor activity; protein binding; syntaxin binding

Biological Process: synaptic vesicle fusion to presynaptic membrane; protein transport; histamine secretion by mast cell; vesicle targeting; post-Golgi vesicle-mediated transport; synaptic vesicle priming