b2-Microglobulin Western Blot Control Protein
b2-Microglobulin, Human Western Blot Control (B2M)
Applications
Western Blot
Purity
Highly PurifiedHighly purified
Synonyms
b2-Microglobulin Western Blot Control; b2-Microglobulin; Human Western Blot Control (B2M); b2-Microglobulin Western Blot Control protein
Host
Human
Specificity
Human b2-microglobulin. Species Sequence Homology is similar to mouse and rat b2-microglobulin.
Purity/Purification
Highly Purified
Highly purified
Highly purified
Form/Format
Supplied as a liquid in SDS-PAGE sample buffer (reduced).
Applicable Applications for b2-Microglobulin Western Blot Control protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot only.
Dilution: Western Blot: 10ul/lane
Optimal dilutions to be determined by researcher.
Dilution: Western Blot: 10ul/lane
Optimal dilutions to be determined by researcher.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for b2-Microglobulin Western Blot Control protein
Positive control for Beta-2-microglobulin M3890-55 and M3890-55A. Purified human B2M protein is formulated in SDS-PAGE sample buffer (reduced) to be used as a positive control for various anti-B2M antibodies using Western Blot. It is ready to be loaded on gels after heating.
Beta-2-microglobulin (B2M) is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning, and other conditions leading to renal tubular dysfunction. Like immunoglobulins, prealbumin, and the beta protein found in the amyloid of Alzheimer disease, B2M has a predominantly beta-pleated sheet structure that may adopt the fibrillar configuration of amyloid in certain pathologic states. The protein is a single polypeptide chain of molecular weight 11,600 (human 119 aa, chromosome 15). B2M associates with heavy hcain of class I MHC antigens on the cell surface. A transient complex of MHC-heavy chain and B2M is is known to be assembled into the TAP molecule involving interation with a number of chaperones. Binding of the processed peptide releases the class I-B2M complex to the cell surface. Absence of binding leads to degradation in the proteasome. Progressive hepatic iron overload, indistinguishable from that observed in HFE, was found only in mice homozygous for the mutated B2M gene.
Beta-2-microglobulin (B2M) is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning, and other conditions leading to renal tubular dysfunction. Like immunoglobulins, prealbumin, and the beta protein found in the amyloid of Alzheimer disease, B2M has a predominantly beta-pleated sheet structure that may adopt the fibrillar configuration of amyloid in certain pathologic states. The protein is a single polypeptide chain of molecular weight 11,600 (human 119 aa, chromosome 15). B2M associates with heavy hcain of class I MHC antigens on the cell surface. A transient complex of MHC-heavy chain and B2M is is known to be assembled into the TAP molecule involving interation with a number of chaperones. Binding of the processed peptide releases the class I-B2M complex to the cell surface. Absence of binding leads to degradation in the proteasome. Progressive hepatic iron overload, indistinguishable from that observed in HFE, was found only in mice homozygous for the mutated B2M gene.
Product Categories/Family for b2-Microglobulin Western Blot Control protein
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Product Notes
The b2-Microglobulin Western Blot Control (Catalog #AAA634785) is a Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's b2-Microglobulin Western Blot Control can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot only. Dilution: Western Blot: 10ul/lane Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the b2-Microglobulin Western Blot Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "b2-Microglobulin Western Blot Control, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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