Thrombin, gamma Protein | F2 protein
Thrombin, gamma, Human
Gene Names
F2; prothrombin
Purity
PurifiedHuman g-thrombin is prepared from purified a-thrombin by limited proteolysis with TPCK-treated trypsin, essentially by the method of Braun et al. (5). Purity is assessed by SDS-PAGE and activity is assessed using a fibrinogen clotting assay.
Synonyms
Thrombin; gamma; Human; F2 protein
Host
Human plasma
Purity/Purification
Purified
Human g-thrombin is prepared from purified a-thrombin by limited proteolysis with TPCK-treated trypsin, essentially by the method of Braun et al. (5). Purity is assessed by SDS-PAGE and activity is assessed using a fibrinogen clotting assay.
Human g-thrombin is prepared from purified a-thrombin by limited proteolysis with TPCK-treated trypsin, essentially by the method of Braun et al. (5). Purity is assessed by SDS-PAGE and activity is assessed using a fibrinogen clotting assay.
Form/Format
Supplied as a liquid in100mM sodium phosphate, 0.1% PEG, pH 6.5.
Extinction Coefficient
E1%1cm, 280nm=18.3
Structure
Four chains (A, B1, B5, B4) with a disulfide link between the A peptide and the B5 peptide.
Preparation and Storage
For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Related Product Information for F2 protein
Alpha-thrombin is a highly specific serine protease generated by proteolytic activation of the zymogen prothrombin (1). Purified forms of a-thrombin have been shown (2-4) to undergo autolysis upon long term storage to less active forms. Similar inactivation was observed upon limited digestion of a-thrombin with trypsin (5). These proteolyzed forms of a-thrombin have been termed b-thrombin and g-thrombin. b-thrombin is produced by the cleavage of the Arg-70 or the Arg-73 bond in the thrombin B-chain. Another form of proteolyzed thrombin, termed b'-thrombin is formed by the single cleavage of thrombin at Arg-154. g-Thrombin is produced by proteolytic cleavage at both of these sites (Arg70/73 and Arg-154) in the B-chain. These cleavages cause release of peptides that are no longer covalently attached to the thrombin molecule, but remain associated through ion exchange and gel filtration chromatography. These proteolyzed forms of thrombin retain their ability to cleave small synthetic substrates (6,7) and some protein substrates such as factor XIII (8), antithrombin III (9) and prothrombin (10). Their ability to clot fibrinogen (11), cleave thrombospondin (12) or activate protein C (9) have been markedly decreased.
Product Categories/Family for F2 protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,300
NCBI Official Full Name
thrombin
NCBI Official Synonym Full Names
coagulation factor II (thrombin)
NCBI Official Symbol
F2
NCBI Official Synonym Symbols
prothrombin
NCBI Protein Information
prothrombin
Protein Family
Uniprot Description
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
Research Articles on F2
Similar Products
Product Notes
The F2 (Catalog #AAA634647) is a Protein produced from Human plasma and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Thrombin, gamma, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
