Western Blot (WB)
(RHOF Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RHOF antibody detected the RHOF protein (arrow).)
Rabbit anti-Human RHOF Polyclonal Antibody | anti-RHOF antibody
RHOF, CT (RHOF, ARHF, RIF, Rho-related GTP-binding protein RhoF, Rho family GTPase Rif, Rho in filopodia) (PE)
Gene Names
RHOF; RIF; ARHF
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RHOF; Polyclonal Antibody; CT (RHOF; ARHF; RIF; Rho-related GTP-binding protein RhoF; Rho family GTPase Rif; Rho in filopodia) (PE); Rho in filopodia; anti-RHOF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-RHOF antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
RHOF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the C-terminal region of human RHOF.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(RHOF Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RHOF antibody detected the RHOF protein (arrow).)
Western Blot (WB)
(RHOF Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RHOF antibody detected the RHOF protein (arrow).)
Related Product Information for anti-RHOF antibody
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.
Product Categories/Family for anti-RHOF antibody
References
Fan, L., et al. J. Cell. Sci. 123 (PT 8), 1247-1252 (2010): Carbone, A., et al. Genes Chromosomes Cancer 47(12):1067-1075(2008). Ellis, S., et al. Curr. Biol. 10(21):1387-1390(2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,869 Da
NCBI Official Full Name
rho-related GTP-binding protein RhoF
NCBI Official Synonym Full Names
ras homolog family member F, filopodia associated
NCBI Official Symbol
RHOF
NCBI Official Synonym Symbols
RIF; ARHF
NCBI Protein Information
rho-related GTP-binding protein RhoF
UniProt Protein Name
Rho-related GTP-binding protein RhoF
Protein Family
UniProt Gene Name
RHOF
UniProt Synonym Gene Names
ARHF; RIF
UniProt Entry Name
RHOF_HUMAN
Research Articles on RHOF
Similar Products
Product Notes
The RHOF rhof (Catalog #AAA6341960) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RHOF, CT (RHOF, ARHF, RIF, Rho-related GTP-binding protein RhoF, Rho family GTPase Rif, Rho in filopodia) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RHOF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RHOF rhof for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RHOF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
