Rabbit anti-Human RASSF8 Polyclonal Antibody | anti-RASSF8 antibody

RASSF8, NT (RASSF8, C12orf2, Ras association domain-containing protein 8, Carcinoma-associated protein HOJ-1) (FITC)

Cat. Num. AAA6340534

• Gene Names: RASSF8; HOJ1; C12orf2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RASSF8; Polyclonal Antibody; NT (RASSF8; C12orf2; Ras association domain-containing protein 8; Carcinoma-associated protein HOJ-1) (FITC); Carcinoma-associated protein HOJ-1; anti-RASSF8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RASSF8 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RASSF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the N-terminal region of human RASSF8.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RASSF8 antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RASSF8 antibody detected the RASSF8 protein (arrow).)

Flow Cytometry (FC/FACS)

(RASSF8 antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-RASSF8 antibody

Antibodies; Cancer Markers

References

Lock, F.E., et al. Oncogene 29(30):4307-4316(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Falvella, F.S., et al. Lung Cancer 56(1):1-7(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Falvella, F.S., et al. Oncogene 25(28):3934-3938(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 258613931
• NCBI GeneID: 11228
• NCBI Accession #: NP_001158219.1
• NCBI GenBank Nucleotide #: NM_001164747.1
• UniProt Accession #: Q8NHQ8; O95647; Q5SCI2; Q76KB6; A8K1Z0
• Molecular Weight: 45,446 Da
• NCBI Official Full Name: ras association domain-containing protein 8 isoform a
• NCBI Official Synonym Full Names: Ras association domain family member 8
• NCBI Official Symbol: RASSF8
• NCBI Official Synonym Symbols: HOJ1; C12orf2
• NCBI Protein Information: ras association domain-containing protein 8
• UniProt Protein Name: Ras association domain-containing protein 8
• Protein Family: Ras association domain-containing protein
• UniProt Gene Name: RASSF8
• UniProt Synonym Gene Names: C12orf2
• UniProt Entry Name: RASF8_HUMAN

NCBI Description

This gene encodes a member of the Ras-assocation domain family (RASSF) of tumor suppressor proteins. This gene is essential for maintaining adherens junction function in epithelial cells and has a role in epithelial cell migration. It is a lung tumor suppressor gene candidate. A chromosomal translocation t(12;22)(p11.2;q13.3) leading to the fusion of this gene and the FBLN1 gene is found in a complex type of synpolydactyly. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]